After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!

Быстрый заказ

Text Size:AAA

Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Human IL11RA Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_004512.3
Размер кДНК:1269bp
Описание кДНК:Full length Clone DNA of Homo sapiens interleukin 11 receptor, alpha (IL11RA), transcript variant 1 with Flag tag.
Синоним гена:IL11RA, MGC2146
Виды:Human
переносчик:pCMV2-FLAG
Plasmid:pCMV2-IL11RA-flag
Участок рестрикции:KpnI + XhoI (5.4kb + 1.32kb)
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
pCMV2-FLAG Vector Information
 
Vector Name pCMV2-FLAG
Vector Size 5592bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag FLAG
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

Schematic of pCMV2-FLAG Multiple Cloning Sites

FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG10252-ACGRBS15400
Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG10252-ACRRBS15400
Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG10252-CFRBS13340
Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG10252-CHRBS13340
Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG10252-CMRBS13340
Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG10252-CYRBS13340
Человек IL11RA/IL-11RA transcript variant 1 Джин клон кДНК в вектор клонированияHG10252-MRBS5130
Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG10252-M-FRBS13340
Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG10252-NFRBS13340
Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG10252-NHRBS13340
Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG10252-NMRBS13340
Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG10252-NYRBS13340
Человек IL11RA/IL-11RA transcript variant 1 Джин ORF экспрессии кДНК клона плазмидыHG10252-UTRBS13340
 Узнайте больше о векторов экспрессии,
Product nameProduct name
Фон

Interleukin 11 receptor, alpha subunit (IL11RA/IL-11RA) is a subunit of the interleukin 11 receptor which is a member of the hematopoietic cytokine receptor family. IL11RA/IL-11RA is expressed in a number of cell lines, including the myelogenous leukemia cell line K562, the megakaryocytic leukemia cell line Mo7E, the erythroleukemia cell line TF1, and the osteosarcoma cell lines, MG-63 and Saos-2. It is also expressed in normal and malignant prostate epithelial cell lines. Expression levels are increased in prostate carcinoma. This particular receptor is very similar to ciliary neurotrophic factor, since both contain an extracellular region with a 2-domain structure composed of an immunoglobulin-like domain and a cytokine receptor-like domain. Alternative splicing has been observed at this locus, and three variants encoding two different isoforms have been identified. IL11RA/IL-11RA is a receptor for interleukin-11. The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. Defects in IL11RA/IL-11RA are a cause of craniosynostosis and dental anomalies (CRSDA). CRSDA is a disorder characterized by craniosynostosis, maxillary hypoplasia, and dental anomalies, including malocclusion, delayed and ectopic tooth eruption, and/or supernumerary teeth. Some patients also display minor digit anomalies, such as syndactyly and/or clinodactyly.

Ссылки
  • Van Leuven F, et al. (1996) Molecular cloning and characterization of the human interleukin-11 receptor alpha-chain gene, IL11RA, located on chromosome 9p13. Genomics. 31 (1): 65-70.
  • Yoshizaki A, et al. (2006) Expression of interleukin (IL)-11 and IL-11 receptor in human colorectal adenocarcinoma: IL-11 up-regulation of the invasive and proliferative activity of human colorectal carcinoma cells. Int J Oncol. 29 (4): 869-76.
  • Karube K, et al. (2006) Gene expression profile of cytokines and chemokines in microdissected primary Hodgkin and Reed-Sternberg (HRS) cells: high expression of interleukin-11 receptor alpha. Ann Oncol. 17 (1): 110-6.
  • Size / Price
    Каталог: HG10252-M-F
    Цена по прейскуранту: 
    Цена:      (You Save: )
    Размер:
    Количество:+-
    НаличиеIn Stock
    Запрос по оптовому заказуДобавить в корзину
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Внимание: все продукты «ПРЕДНАЗНАЧЕНЫ ИСКЛЮЧИТЕЛЬНО ДЛЯ ИССЛЕДОВАНИЙ И НЕ ПРЕДНАЗНАЧЕНЫ ДЛЯ ДИАГНОСТИКИ ИЛИ ТЕРАПЕВТИЧЕСКОГО ЛЕЧЕНИЯ».