Anti-IL1-F8 Antibody

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Anti-IL1-F8 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-IL1-F8 Antibody
Validated applications
WB,ELISA,IHC-P
Species reactivity
Reacts with: Mouse
Specificity
Mouse IL1-F8
Immunogen
Recombinant Mouse IL-36B / IL-1F8 protein (Catalog#51071-MNAE)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse IL-36B / IL-1F8 (rM IL-36B / IL-1F8; Catalog#51071-MNAE; Q9D6Z6; Met1-Lys183). IL-36B / IL-1F8 specific IgG was purified by Mouse IL-36B / IL-1F8 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IL1-F8 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:500-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IL1-F8 Antibody (Rabbit Polyclonal antibody) Images

Immunochemical staining of mouse IL1F8 in mouse stomach with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining of mouse IL1F8 in mouse intestine with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

Anti-IL1F8 rabbit polyclonal antibody at 1:500 dilution

Lane A: 293T Membrane Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:19 kDa

Observed band size:16 kDa

(We are unsure as to the identity of these extra bands.)

Anti-IL1-F8 Antibody: Alternative Names

Anti-2310043N20Rik Antibody; Anti-Il36b Antibody; Anti-RP23-176J12.1 Antibody

IL1-F8 Background Information

Interleukin-1 family member 8(IL1F8) also known as IL36B, is a member of the interleukin 1(IL-1) cytokine family. IL1F8 may contains a 12-stranded beta-trefoil structure. Until recently, the IL-1 family of cytokines includ four members, with three having pro-inflammatory effects such as IL1F8 and the fourth member being an IL-1 receptor antagonist (IL-1Ra). IL-1 family members exert their effects through binding to receptors that belong to the IL-1 receptor (IL-1R) family. IL1F8 exerts proinflammatory effects in primary human joint cells. Joint and serum IL-1F8 protein levels did not correlate with inflammation, but they were high in some human serum samples tested, including samples from patients with rheumatoid arthritis. IL1F8 also activated NK-κB.
Full Name
interleukin 36, beta
References
  • Magne D, et al. (2006) The new IL-1 family member IL-1F8 stimulates production of inflammatory mediators by synovial fibroblasts and articular chondrocytes. Arthritis Research & Therapy. 8: R80.
  • Smith DE, et al. (2000) The new IL-1 family member IL-1F8 stimulates production of inflammatory mediators by synovial fibroblasts and articular chondrocytes. The Journal of Biological Chemistry. 275: 1169-75.
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