VEGF B cDNA ORF Clone, Human, N-DDK (Flag®) tag

Cat: HG10544-NF
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VEGF B cDNA ORF Clone, Human, N-DDK (Flag®) tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
624 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human vascular endothelial growth factor B with N terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-SP-N-FLAG
Restriction Sites
HindIII + XbaI (6kb + 0.65kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
VEGF B cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

VEGF B cDNA ORF Clone, Human, N-DDK (Flag®) tag Validated Images
Cynomolgus CD19/B4/CVID3 Gene Plasmid Map 5623
Human VEGF-B Gene Expression validated Image 14448
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
VEGF B cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names
VEGFL cDNA ORF Clone, Human;VRF cDNA ORF Clone, Human
VEGF B Background Information
Full Name
vascular endothelial growth factor B

VEGF B cDNA ORF Clone, Rat, Related Products

Product Name Catalog#(PDF)
VEGF B cDNA ORF Clone in Cloning Vector, Rat RG80114-G
VEGF B cDNA ORF Clone, Rat, untagged RG80114-UT
VEGF B cDNA ORF Clone, Rat, C-GFPSpark® tag RG80114-ACG
VEGF B Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80114-ACGLN
VEGF B cDNA ORF Clone, Rat, C-OFPSpark® tag RG80114-ACR
VEGF B cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80114-CF
VEGF B cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80114-NF
VEGF B cDNA ORF Clone, Rat, C-His tag RG80114-CH
VEGF B cDNA ORF Clone, Rat, N-His tag RG80114-NH
VEGF B cDNA ORF Clone, Rat, C-HA tag RG80114-CY
VEGF B cDNA ORF Clone, Rat, N-HA tag RG80114-NY
VEGF B cDNA ORF Clone, Rat, C-Myc tag RG80114-CM
VEGF B cDNA ORF Clone, Rat, N-Myc tag RG80114-NM
VEGF B qPCR Primer Pairs, Rat RP300109

VEGF B cDNA ORF Clone, Mouse, Related Products

Product Name Catalog#(PDF)
VEGF B cDNA ORF Clone in Cloning Vector, Mouse MG50158-M
VEGF B cDNA ORF Clone, Mouse, untagged MG50158-UT
VEGF B Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50158-ACGLN
VEGF B cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50158-ACG
VEGF B cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50158-ACR
VEGF B cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50158-CF
VEGF B cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50158-NF
VEGF B cDNA ORF Clone, Mouse, C-His tag MG50158-CH
VEGF B cDNA ORF Clone, Mouse, N-His tag MG50158-NH
VEGF B cDNA ORF Clone, Mouse, C-HA tag MG50158-CY
VEGF B cDNA ORF Clone, Mouse, N-HA tag MG50158-NY
VEGF B cDNA ORF Clone, Mouse, C-Myc tag MG50158-CM
VEGF B cDNA ORF Clone, Mouse, N-Myc tag MG50158-NM
VEGF B qPCR Primer Pairs, Mouse MP200188

VEGF B cDNA ORF Clone, Human, Related Products

Product Name Catalog#(PDF)
VEGF B cDNA ORF Clone in Cloning Vector, Human HG10544-M
VEGF B cDNA ORF Clone, Human, untagged HG10544-UT
VEGF B cDNA ORF Clone, Human, C-GFPSpark® tag HG10544-ACG
VEGF B Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10544-ACGLN
VEGF B cDNA ORF Clone, Human, C-OFPSpark® tag HG10544-ACR
VEGF B cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10544-CF
VEGF B cDNA ORF Clone, Human, C-His tag HG10544-CH
VEGF B cDNA ORF Clone, Human, N-His tag HG10544-NH
VEGF B cDNA ORF Clone, Human, C-HA tag HG10544-CY
VEGF B cDNA ORF Clone, Human, N-HA tag HG10544-NY
VEGF B cDNA ORF Clone, Human, C-Myc tag HG10544-CM
VEGF B cDNA ORF Clone, Human, N-Myc tag HG10544-NM
VEGF B qPCR Primer Pairs, Human HP100560
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