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Человек TNFSF13 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка

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Human TNFSF13 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_003808.3
Размер кДНК:753bp
Описание кДНК:Full length Clone DNA of Homo sapiens tumor necrosis factor (ligand) superfamily, member 13 with C terminal Myc tag.
Синоним гена:APRIL, CD256, TALL2, TRDL-1, ligand, UNQ383/PRO715
Виды:Human
переносчик:pCMV3-C-Myc
Plasmid:pCMV3-TNFSF13-Myc
Участок рестрикции:KpnI + XbaI (6kb + 0.8kb)
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Human TNFSF13 Gene Plasmid Map
Human TNFSF13 natural ORF mammalian expression plasmid, C-Myc tag
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Человек TNFSF13 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка on other vectors
Человек TNFSF13 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG10610-ACGRBS15400
Человек TNFSF13 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG10610-ACRRBS15400
Человек TNFSF13 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG10610-CFRBS13340
Человек TNFSF13 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG10610-CHRBS13340
Человек TNFSF13 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG10610-CMRBS13340
Человек TNFSF13 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG10610-CYRBS13340
Человек TNFSF13 Джин клон кДНК в вектор клонированияHG10610-MRBS5130
Человек TNFSF13 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG10610-NFRBS13340
Человек TNFSF13 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG10610-NHRBS13340
Человек TNFSF13 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG10610-NMRBS13340
Человек TNFSF13 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG10610-NYRBS13340
Человек TNFSF13 Джин ORF экспрессии кДНК клона плазмидыHG10610-UTRBS13340
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Фон

TNFSF13 is a member of the tumor necrosis factor (TNF) ligand family. It is a ligand for TNFRSF17/BCMA. TNFSF13 is lowly expressed in normal tissues, but is elevated in several types of tumors and transformed cell lines. It is mportant for B cell development. TNFSF13 may also play a role in T-independent type II antigen responses and T cell survival, and induce proliferation/survival of non lymphoid cells. It exists as a functional homotrimer. It can bind to two cell surface receptors, BCMA and TACI, which it shares with BAFF to exert downstream T- and B-cell regulatory effects. TNFSF13 also has been demonstrated to bind to proteoglycans on the cell surface.

Ссылки
  • Bossen C, et al. (2007) BAFF, APRIL and their receptors: structure, function and signaling. Semin. Immunol. 18(5):263-75.
  • Tangye SG, et al. (2007) BAFF, APRIL and human B cell disorders. Immunol. 18(5): 305-17.
  • Osman W, et al. (2012) Association of common variants in TNFRSF13B, TNFSF13, and ANXA3 with serum levels of non-albumin protein and immunoglobulin isotypes in Japanese. PLoS One. 7(4):e32683.
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    Каталог: HG10610-CM
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