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Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

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Человек SLITRK1 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_052910.1
Размер кДНК:2091bp
Описание кДНК:Full length Clone DNA of Homo sapiens SLIT and NTRK-like family, member 1 with C terminal Flag tag.
Синоним гена:LRRC12, FLJ54428, KIAA0918, KIAA1910, RP11-395N17.1
Виды:Human
переносчик:pCMV3-C-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with SLITRK1 qPCR primers for gene expression analysis, HP100378 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG10340-ACGRBS16760
Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG10340-ACRRBS16760
Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG10340-CFRBS14710
Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG10340-CHRBS14710
Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG10340-CMRBS14710
Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG10340-CYRBS14710
Человек SLITRK1 Джин клон кДНК в вектор клонированияHG10340-MRBS5130
Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмидыHG10340-M-NRBS14710
Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG10340-NFRBS14710
Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG10340-NHRBS14710
Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG10340-NMRBS14710
Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG10340-NYRBS14710
Человек SLITRK1 Джин ORF экспрессии кДНК клона плазмидыHG10340-UTRBS14710
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Product nameProduct name
Фон

SLITRK1 (Slit and Trk-like family member 1) is a integral membrane protein belonging to the SLITRK family consists of at least 6 members (SLITRK1-6). They are named and characterized by the presence of two leucine-rich repeats (LRRs) in the extracellular domain similar to those found in a secreted axonal growth-controlling protein, Slit, as well as a C-terminal domain with homology to Trk neurotrophin tyrosine kinase receptors. Expression of SLITRKs are highly restricted to neural tissues, and are identified as the neuronal components modulating the neurite outgrowth. More specifically, SLITRK1 expression is found in the mature neurons of the cerebrum, thalamus and hippocampus, and induces unipolar neurites in cultured neuronal cells. Human SLITRK1 is a 696 amino acid precursor protein, and one truncating frameshift mutation (448 aa) has been linked to Tourette's syndrome, a genetically influenced developmental neuropsychiatric disorder characterized by chronic vocal and motor tics. In addition, all SLITRK genes are differentially expressed in brain tumors, such as astrocytoma, oligodendroglioma, glioblastoma, and are suggested to be useful molecular indicators of brain tumor properties.

Ссылки

1. Aruga, J. and Mikoshiba, K. 2003, Mol. Cell. Neurosci. 24: 117-129.

2. Aruga, J. et al., 2003, Gene. 315: 87-94.

3. Abelson, J.F. et al., 2005, Science. 310: 317-320.

4. Grados, M.A. and Walkup. J.T. 2006, Trends. Genet. 22: 291-293.

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Каталог: HG10340-CF
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