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Человек S100A12 Джин ORF экспрессии кДНК клона плазмиды, C-HA Метка

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Human S100A12 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_005621.1
Размер кДНК:279bp
Описание кДНК:Full length Clone DNA of Homo sapiens S100 calcium binding protein A5 with C terminal HA tag.
Синоним гена:p6, CAGC, CGRP, MRP6, CAAF1, ENRAGE, S100A12
Виды:Human
переносчик:pCMV3-C-HA
Plasmid:
Участок рестрикции:
Последовательность меток:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Человек S100A12 Джин ORF экспрессии кДНК клона плазмиды, C-HA Метка on other vectors
Человек S100A12 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG11143-ACGRBS15400
Человек S100A12 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG11143-ACRRBS15400
Человек S100A12 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG11143-CFRBS13340
Человек S100A12 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG11143-CHRBS13340
Человек S100A12 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG11143-CMRBS13340
Человек S100A12 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG11143-CYRBS13340
Человек S100A12 Джин клон кДНК в вектор клонированияHG11143-MRBS5130
Человек S100A12 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG11143-M-FRBS13340
Человек S100A12 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG11143-NFRBS13340
Человек S100A12 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG11143-NHRBS13340
Человек S100A12 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG11143-NMRBS13340
Человек S100A12 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG11143-NYRBS13340
Человек S100A12 Джин ORF экспрессии кДНК клона плазмидыHG11143-UTRBS13340
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Фон

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. 

Protein S100-A12, also known as S100 calcium-binding protein A12, Calcium-binding protein in amniotic fluid 1, Calgranulin-C, and S100A12, is a member of the S-101 family. Like the majority of S100 proteins, S100A12 is a dimer, with the interface between the two subunits being composed mostly of hydrophobic residues. The fold of S100A12 is similar to the other known crystal and solution structures of S100 proteins, except for the linker region between the two EF-hand motifs. S100A12 plays an important role in the inflammatory response.

Ссылки
  • Moroz, OV. et al., 2001, Acta Crystallogr D Biol Crystallogr. 57: 20-9.
  • Foell, D. et al., 2003, Lancet  361 (9365):1270-2.
  • Vogl, T. et al., 2004, Blood. 104: 4260-8.
  • Viemann, D. et al., 2005, Blood. 105: 2955-62.
  • Nakatani, Y. et al., 2005, Mediators Inflamm. 2005: 280-292.
  • Bjoerk, P. et al., 2009, PLoS Biol. 7: E97-E97.
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    Каталог: HG11143-CY
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    Количество:+-
    Наличие2-3 weeks
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