|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human RBP4 ORF mammalian expression plasmid, C-GFPSpark tag||HG10354-ACG|
|Human RBP4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10354-ACR|
|Human RBP4 ORF mammalian expression plasmid, C-Flag tag||HG10354-CF|
|Human RBP4 ORF mammalian expression plasmid, C-His tag||HG10354-CH|
|Human RBP4 ORF mammalian expression plasmid, C-Myc tag||HG10354-CM|
|Human RBP4 ORF mammalian expression plasmid, C-HA tag||HG10354-CY|
|人 RBP4 基因全长ORF克隆||HG10354-M|
|Human RBP4 ORF mammalian expression plasmid, N-Flag tag||HG10354-NF|
|Human RBP4 ORF mammalian expression plasmid, N-His tag||HG10354-NH|
|Human RBP4 ORF mammalian expression plasmid, N-Myc tag||HG10354-NM|
|Human RBP4 ORF mammalian expression plasmid, N-HA tag||HG10354-NY|
|Human RBP4 natural ORF mammalian expression plasmid||HG10354-UT|
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Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM).