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Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, N-Myc Метка

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Human PTP4A2 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:BC070182
Размер кДНК:504bp
Описание кДНК:Full length Clone DNA of Homo sapiens protein tyrosine phosphatase type IVA, member 2 with N terminal Myc tag.
Синоним гена:HH13, OV-1, PRL2, HH7-2, PRL-2, PTP4A, HU-PP-1, PTPCAAX2, ptp-IV1a, ptp-IV1b
Виды:Human
переносчик:pCMV3-N-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, N-Myc Метка on other vectors
Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG13097-ACGRBS15400
Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG13097-ACRRBS15400
Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаHG13097-ANGRBS15400
Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаHG13097-ANRRBS15400
Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG13097-CFRBS13340
Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG13097-CHRBS13340
Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG13097-CMRBS13340
Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG13097-CYRBS13340
Человек PRL-2/PTP4A2 Джин клон кДНК в вектор клонированияHG13097-GRBS5130
Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG13097-NFRBS13340
Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG13097-NHRBS13340
Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG13097-NMRBS13340
Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG13097-NYRBS13340
Человек PRL-2/PTP4A2 Джин ORF экспрессии кДНК клона плазмидыHG13097-UTRBS13340
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Фон

PRL-2 (Protein-tyrosine phosphatase of regenerating liver 2), also known as PTP4A2 (Protein tyrosine phosphatase type IVA, member 2), is a member of PTP family and has an important function in controlling cell growth. PRL-2 phosphatases may be multifunctional enzymes with diverse roles in a variety of tissue and cell types. The phosphatase of regenerating liver (PRL) family, comprising PRL-1, PRL-2 and PRL-3, is a group of prenylated phosphatases that are candidate cancer biomarkers and therapeutic targets. PRL-1, PRL-2, and PRL-3 represent a novel class of protein-tyrosine phosphatase with a C-terminal prenylation motif. They are three closely related intracellular enzymes that possess the PTP active site signature sequence CX 5R. The PRL-2 mRNA is elevated in primary breast tumors relative to matched normal tissue, and also dramatically elevated in metastatic lymph nodes compared with primary tumors. PRL-2 plays a role in breast cancer progression. PRL-2 is a pathogenic molecule in hematopoietic malignancies and suggest its potential as a novel therapeutic target.

Ссылки
  • Akiyama S, et al. (2010) PRL-2 increases Epo and IL-3 responses in hematopoietic cells. Blood Cells Mol Dis. 44(4): 209-14.
  • Hardy S, et al. (2010) Overexpression of the protein tyrosine phosphatase PRL-2 correlates with breast tumor formation and progression. Cancer Res. 70(21): 8959-67.
  • Yuan L, et al. (2007) Differential expression and functional constraint of PRL-2 in hibernating bat. Comp Biochem Physiol B Biochem Mol Biol. 148(4): 375-81.
  • Dumaual CM, et al. (2006) Cellular localization of PRL-1 and PRL-2 gene expression in normal adult human tissues. J Histochem Cytochem. 54(12): 1401-12.
  • Zeng Q, et al. (2000) Prenylation-dependent association of protein-tyrosine phosphatases PRL-1, -2, and -3 with the plasma membrane and the early endosome. J Biol Chem. 275(28): 21444-52.
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