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Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, N-Myc Метка

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Human PKM Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_002654.3
Размер кДНК:1596bp
Описание кДНК:Full length Clone DNA of Homo sapiens pyruvate kinase, muscle with N terminal Myc tag.
Синоним гена:PK3, PKM, TCB, OIP3, CTHBP, THBP1, MGC3932, PKM2
Виды:Human
переносчик:pCMV3-N-Myc
Plasmid:pCMV3-Myc-PKM
Участок рестрикции:KpnI + NotI (6kb + 1.65kb)
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Human PKM Gene Plasmid Map
Human PKM2 natural ORF mammalian expression plasmid, N-Myc tag
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, N-Myc Метка on other vectors
Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG11430-ACGRBS16760
Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG11430-ACRRBS16760
Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаHG11430-ANGRBS16760
Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаHG11430-ANRRBS16760
Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG11430-CFRBS14710
Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG11430-CHRBS14710
Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG11430-CMRBS14710
Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG11430-CYRBS14710
Человек PKM2 Джин клон кДНК в вектор клонированияHG11430-MRBS5130
Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG11430-NFRBS14710
Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG11430-NHRBS14710
Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG11430-NMRBS14710
Человек PKM2 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG11430-NYRBS14710
Человек PKM2 Джин ORF экспрессии кДНК клона плазмидыHG11430-UTRBS14710
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Фон

Pyruvate kinase isozymes M2 also known as pyruvate kinase muscle isozyme 2 (PKM2), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an isoenzyme of the glycolytic enzyme pyruvate kinase. Pyruvate kinase isozymes M2 / PKM2 is a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. PKM2 has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. PKM2 functions as a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. PKM2 may stimulates POU5F1-mediated transcriptional activation. This protein Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms of PKM2 contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Ссылки
  • Bluemlein K, et al. (2011) No evidence for a shift in pyruvate kinase PKM1 to PKM2 expression during tumorigenesis. Oncotarget. 2 (5): 393-400.
  • Gupta V, et al. (2010) Dominant Negative Mutations Affect Oligomerization of Human Pyruvate Kinase M2 Isozyme and Promote Cellular Growth and Polyploidy. J Biol Chem. 285 (22): 16864-73.
  • Eigenbrodt E, et al. (1992) Double role for pyruvate kinase type M2 in the expansion of phosphometabolite pools found in tumor cells. Crit Rev Oncog. 3 (1): 91-115.
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    Каталог: HG11430-NM
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