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Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

    ПаспортОбзорыСвязанные продуктыПротоколы
    Человек PARVA Информация о продукте «Клон cDNA»
    Gene_bank_ref_id:BC016713
    Размер кДНК:1119bp
    Описание кДНК:Full length Clone DNA of Homo sapiens parvin, alpha with C terminal Flag tag.
    Синоним гена:MXRA2, CH-ILKBP
    Виды:Human
    переносчик:pCMV3-C-FLAG
    Plasmid:
    Участок рестрикции:
    Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    Описание последовательности:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with PARVA qPCR primers for gene expression analysis, HP102583 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Склад:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG13919-ACGRBS15400
    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG13919-ACRRBS15400
    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаHG13919-ANGRBS15400
    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаHG13919-ANRRBS15400
    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG13919-CFRBS13340
    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG13919-CHRBS13340
    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG13919-CMRBS13340
    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG13919-CYRBS13340
    Человек Actopaxin Джин клон кДНК в вектор клонированияHG13919-GRBS5130
    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG13919-NFRBS13340
    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG13919-NHRBS13340
    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG13919-NMRBS13340
    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG13919-NYRBS13340
    Человек Actopaxin Джин ORF экспрессии кДНК клона плазмидыHG13919-UTRBS13340
     Узнайте больше о векторов экспрессии,
    Product nameProduct name
    Фон

    Actopaxin, also known as alpha-parvin, belongs to the parvin family. It is widely expressed, with highest levels in heart, skeletal muscle, kidney and liver. Actopaxin contains 2 CH (calponin-homology) domains and probably plays a role in the regulation of cell adhesion and cytoskeleton organization. It interacts with integrin-linked protein kinase and probably with actin and the LD1 and LD4 motifs of PXN. Actopaxin binds directly to both F-actin and paxillin LD1 and LD4 motifs. Actopaxin also exhibits robust focal adhesion localization in several cultured cell types but is not found along the length of the associated actin-rich stress fibers. It is absent from actin-rich cell-cell adherens junctions.

    Ссылки
  • Korenbaum E, et al. (2002) Genomic organization and expression profile of the parvin family of focal adhesion proteins in mice and humans. Gene. 279(1):69-79.
  • Nikolopoulos SN, et al. (2002) Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization. J Biol Chem. 277(2): 1568-75.
  • Tu Y, et al. (2001) A new focal adhesion protein that interacts with integrin-linked kinase and regulates cell adhesion and spreading. J Cell Biol. 153(3): 585-98.
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    Каталог: HG13919-CF
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