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Человек NKp80 / KLRF1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Human KLRF1 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_016523.1
Размер кДНК:696bp
Описание кДНК:Full length Clone DNA of Homo sapiens Killer ell lectin-like receptor subfamily F, member 1 with C terminal Flag tag.
Синоним гена:CLEC5C, MGC119907, MGC119908, MGC119909
Виды:Human
переносчик:pCMV3-C-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Человек NKp80 / KLRF1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Человек NKp80 / KLRF1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG10984-ACGRBS15400
Человек NKp80 / KLRF1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG10984-ACRRBS15400
Человек NKp80 / KLRF1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG10984-CFRBS13340
Человек NKp80 / KLRF1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG10984-CHRBS13340
Человек NKp80 / KLRF1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG10984-CMRBS13340
Человек NKp80 / KLRF1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG10984-CYRBS13340
Человек NKp80 / KLRF1 Джин клон кДНК в вектор клонированияHG10984-MRBS5130
Человек NKp80 / KLRF1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG10984-NFRBS13340
Человек NKp80 / KLRF1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG10984-NHRBS13340
Человек NKp80 / KLRF1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG10984-NMRBS13340
Человек NKp80 / KLRF1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG10984-NYRBS13340
Человек NKp80 / KLRF1 Джин ORF экспрессии кДНК клона плазмидыHG10984-UTRBS13340
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Product nameProduct name
Фон

NKp80, also known as KLRF1, is an activating homodimeric C-type lectin-like receptor which is expressed on nearly all natural killer cells and stimulates their cytoxicity and cytokine release. NKp80 stimulates cytotoxicity upon engagement of its genetically linked ligand: myeloid-specific CTLR activation-induced C-type lectin (AICL). NKp80, but not NKp80 mutated at tyrosine 7 (NKp80/Y7F), is tyrosine phosphorylated. Accordingly, NKp80/Y7F, but not NKp80/Y30F or NKp80/Y37F, failed to induce cytotoxicity. NKp80 phosphopeptides comprising the hemi-ITAM-like sequence surrounding tyrosine 7 bound Lck- and Syk-family kinases; accordingly, cross-linking of NKp80, but not NKp80/Y7F, induced Syk phosphorylation. Moreover, inhibition of Syk kinase, but not ZAP-70 kinase, impaired cytotoxic responses through NKp80. Atypical residues in the hemi-ITAM-like motif of NKp80 cause an altered stoichiometry of phosphorylation but did not substantially affect NK cytotoxicity. Altogether, these results show that NKp80 uses an atypical hemi-ITAM and Syk kinase to trigger cellular cytotoxicity.

Ссылки
  • Kuttruff S. et al., 2009, Blood. 113 (2): 358-69.
  • Dennehy KM. et al., 2011, J Immunol. 186 (2): 657-61.
  • Roda-Navarro P. et al., 2000, Eur J Immunol. 30 (2): 568-76.
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    Каталог: HG10984-CF
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    Количество:+-
    Наличие2-3 weeks
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