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Человек ICOS/AILIM/CD278 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

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Human ICOS Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_012092.2
Размер кДНК:600bp
Описание кДНК:Full length Clone DNA of Homo sapiens inducible T-cell co-stimulator with C terminal Flag tag.
Синоним гена:AILIM, CD278, MGC39850
Виды:Human
переносчик:pCMV3-C-FLAG
Plasmid:pCMV3-ICOS-Flag
Участок рестрикции:KpnI + NotI (6kb + 0.64kb)
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Human ICOS Gene Plasmid Map
Human ICOS natural ORF mammalian expression plasmid, C-Flag tag
Human ICOS Gene Expression validated Image
Human ICOS ORF mammalian expression plasmid, C-Flag tag
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Caption:
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Человек ICOS/AILIM/CD278 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Человек ICOS/AILIM/CD278 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG10344-ACGRBS15400
Человек ICOS/AILIM/CD278 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG10344-ACRRBS15400
Человек ICOS/AILIM/CD278 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG10344-CFRBS13340
Человек ICOS/AILIM/CD278 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG10344-CHRBS13340
Человек ICOS/AILIM/CD278 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG10344-CMRBS13340
Человек ICOS/AILIM/CD278 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG10344-CYRBS13340
Человек ICOS/AILIM/CD278 Джин клон кДНК в вектор клонированияHG10344-MRBS5130
Человек ICOS/AILIM/CD278 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG10344-NFRBS13340
Человек ICOS/AILIM/CD278 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG10344-NHRBS13340
Человек ICOS/AILIM/CD278 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG10344-NMRBS13340
Человек ICOS/AILIM/CD278 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG10344-NYRBS13340
Человек ICOS/AILIM/CD278 Джин ORF экспрессии кДНК клона плазмидыHG10344-UTRBS13340
 Узнайте больше о векторов экспрессии,
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Фон

Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) is a cell-surface receptor, and belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. The interaction of B7-H2/ICOS plays a critical role in Th cell differentiation, T−B cell interactions which is essential for germinal center formation, and humoral immune responses, and as well as the production of cytokine IL-4. In addition, ICOS is more potent in the induction of IL-10 production, a cytokine important for suppressive function of T regulatory cells. The B7-1/B7-2--CD28/CTLA-4 and ICOS-B7RP-1 pathway provides key second signals that can regulate the activation, inhibition and fine-tuning of T-lymphocyte responses. ICOS stimulates both Th1 and Th2 cytokine production but may have a preferential role in Th2 cell development. Moreover, The B7-1/B7-2-CD28/CTLA-4 and ICOS-B7RP-1 pathway has been suggested of being involved in the development of airway inflammation and airway hyperresponsiveness.

Ссылки
  • Coyle AJ, et al. (2004) The role of ICOS and other costimulatory molecules in allergy and asthma. Springer Semin Immunopathol. 25(3-4): 349-59.
  • Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.
  • van Berkel ME, et al. (2006) CD28 and ICOS: similar or separate costimulators of T cells Immunol Lett. 105(2): 115-22.
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    Каталог: HG10344-CF
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    • Human ICOS natural ORF mammalian expression plasmid, C-Flag tag
    • Human ICOS ORF mammalian expression plasmid, C-Flag tag
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