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Человек HRAS Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка

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Human HRAS Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001130442.1
Размер кДНК:570bp
Описание кДНК:Full length Clone DNA of Homo sapiens v-Ha-ras Harvey rat sarcoma viral oncogene homolog with N terminal Flag tag.
Синоним гена:CTLO, HAMSV, HRAS1, K-RAS, N-RAS, RASH1, C-H-RAS, H-RASIDX, C-BAS/HAS, C-HA-RAS1, HRAS
Виды:Human
переносчик:pCMV3-N-FLAG
Plasmid:pCMV3-Flag-HRAS
Участок рестрикции:KpnI + XbaI (6kb + 0.62kb)
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Human HRAS Gene Plasmid Map
Human HRAS natural ORF mammalian expression plasmid, N-Flag tag
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Человек HRAS Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка on other vectors
Человек HRAS Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG12059-ACGRBS15400
Человек HRAS Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG12059-ACRRBS15400
Человек HRAS Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG12059-CFRBS13340
Человек HRAS Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG12059-CHRBS13340
Человек HRAS Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG12059-CMRBS13340
Человек HRAS Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG12059-CYRBS13340
Человек HRAS Джин клон кДНК в вектор клонированияHG12059-GRBS5130
Человек HRAS Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG12059-NFRBS13340
Человек HRAS Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG12059-NHRBS13340
Человек HRAS Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG12059-NMRBS13340
Человек HRAS Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG12059-NYRBS13340
Человек HRAS Джин ORF экспрессии кДНК клона плазмидыHG12059-UTRBS13340
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Фон

HRas, also known as HRAS, belongs to the small GTPase superfamily, Ras family and is widely expressed. It functions in signal transduction pathways. HRas can bind GTP and GDP, and they have intrinsic GTPase activity. It undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS). FCSS is arare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities, tumor predisposition, skin and musculoskeletal abnormalities. Defects in HRAS also can cause congenital myopathy with excess of muscle spindles. HRAS deficiency may be a cause of susceptibility to Hurthle cell thyroid carcinoma. It has been shown that defects in HRAS can cause susceptibility to bladder cancer which is a malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences. Defects in HRAS are the cause of oral squamous cell carcinoma.

Ссылки
  • Schulten HJ, et al. (2011) Mutational screening of RET, HRAS, KRAS, NRAS, BRAF, AKT1, and CTNNB1 in medullary thyroid carcinoma. Anticancer Res. 31(12):4179-83.
  • Gripp KW, et al. (2011) Molecular confirmation of HRAS p.G12S in siblings with Costello syndrome. Am J Med Genet A. 155A(9):2263-8.
  • Na KY, et al. (2012) Allelic loss of susceptibility loci and the occurrence of BRAF and RAS mutations in patients with familial non-medullary thyroid cancer. J Surg Oncol. 105(1):10-4.
  • Membrino A, et al. (2011) G4-DNA formation in the HRAS promoter and rational design of decoy oligonucleotides for cancer therapy. PLoS One. 6(9):e24421.
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    Каталог: HG12059-NF
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