GFPT1 cDNA ORF Clone, Human, N-DDK (Flag®) tag General Information
Identical with the Gene Bank Ref. ID sequence.
Full length Clone DNA of Human glutamine-fructose-6-phosphate transaminase 1 with N terminal Flag tag.
Enhanced CMV promoter
KpnI + XbaI(6kb+2.09kb)
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
GFPT1 cDNA ORF Clone, Human, N-DDK (Flag®) tag Validated Images
GFPT1 cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names
CMSTA1 cDNA ORF Clone, Human;GFA cDNA ORF Clone, Human;GFAT cDNA ORF Clone, Human;GFAT1 cDNA ORF Clone, Human;GFAT1m cDNA ORF Clone, Human;GFPT cDNA ORF Clone, Human;GFPT1L cDNA ORF Clone, Human;MSLG cDNA ORF Clone, Human
GFPT1 Background Information
Glutamine:fructose-6-phosphate amidotransferase 1 (GFAT), also known as GFPT1, is a member of the N-terminal nucleophile aminotransferases and the first rate-limiting enzyme for the entry of glucose into the hexosamine biosynthesis pathway (HBP) in mammals. GFAT transfers the amino group from the L-glutamine amide to the D-fructose 6-phosphate, producing glutamic acid and glucosamine 6-phosphate. GFAT exists as a homotetramer in cytoplasm, and is proposed to be most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. The full length of human GFAT contains 1 glutamine amidotransferase type-2 domain which catalyzes amide nitrogen transfer from glutamine to the appropriate substrate, and 2 SIS (Sugar Isomerase) domains found in many phosphosugar isomerases and phosphosugar binding proteins.Two isoforms of gfat have been identified: GFAT1 is predominantly expressed in skeletal muscle, whereas GFAT2 is expressed mainly in the central nervous system.
glutamine--fructose-6-phosphate transaminase 1
McKnight G.L., et al.,(1992), Molecular cloning, cDNA sequence, and bacterial expression of human glutamine:fructose-6-phosphate amidotransferase. J. Biol. Chem. 267:25208-25212.Hillier L.W., et al., (2005), Generation and annotation of the DNA sequences of human chromosomes 2 and 4.Nature 434:724-731.Beausoleil S.A., et al.,(2006), A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat. Biotechnol. 24:1285-1292.