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Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, N-HA Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Human GADD45A Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001924.2
Размер кДНК:498bp
Описание кДНК:Full length Clone DNA of Homo sapiens growth arrest and DNA.-damage-inducible, alpha with N terminal HA tag.
Синоним гена:DDIT1, GADD45, GADD45A
Виды:Human
переносчик:pCMV3-N-HA
Plasmid:
Участок рестрикции:
Последовательность меток:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, N-HA Метка on other vectors
Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG11156-ACGRBS15400
Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG11156-ACRRBS15400
Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаHG11156-ANGRBS15400
Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаHG11156-ANRRBS15400
Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG11156-CFRBS13340
Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG11156-CHRBS13340
Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG11156-CMRBS13340
Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG11156-CYRBS13340
Человек GADD45A/DDIT1 Джин клон кДНК в вектор клонированияHG11156-MRBS5130
Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG11156-NFRBS13340
Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG11156-NHRBS13340
Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG11156-NMRBS13340
Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG11156-NYRBS13340
Человек GADD45A/DDIT1 Джин ORF экспрессии кДНК клона плазмидыHG11156-UTRBS13340
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Фон

GADD45A is a member of the GADD45 Family, and has been found to associate with several cytoplasmic and nuclear factors and has been implicated in several cellular functions, including MAPK signaling, cell cycle regulation, DNA repair and genomic stability, apoptosis, and immune responses. The GADD45 Family of genes is rapidly induced by different stressors, including differentiation-inducing cytokines, and there is a large body of evidence that their cognate proteins are key players in cellular stress responses. GADD45A protein has been reported to interact with multiple important cellular proteins, including Cdc2 protein kinase, proliferating cell nuclear antigen (PCNA), p21Waf1/Cip1 protein, core histone protein and MTK/MEKK4, an up-stream activator of the JNK/SAPK pathway, indicating that GADD45A may play important roles in the control of cell cycle checkpoint, DNA repair process, and signaling transduction. GADD45A expression in response to genotoxic stress illustrates a more complex scenario, wherein transcriptional changes operate in concert with mRNA turnover and translational regulation. GADD45A was the first stress-inducible gene determined to be up-regulated by p53 and is also a target for the p53 homologues, p63 and p73. The decreased GADD45A expression is also considered a survival mechanism, as cancer cells without this control can evade the apoptotic pathway leading to increased tumourigenesis. As GADD45A is an essential component of many metabolic pathways that control proliferating cancer cells, it presents itself as an emerging drug target worthy of further investigation.

Ссылки
  • Zhan Q. (2005) Gadd45a, a p53- and BRCA1-regulated stress protein, in cellular response to DNA damage. Mutat Res. 569(1-2): 133-43.
  • Lal A, et al. (2006) Egad, more forms of gene regulation: the gadd45a story. Cell Cycle. 5(13): 1422-5.
  • Hoffman B, et al. (2007) Role of gadd45 in myeloid cells in response to hematopoietic stress. Blood Cells Mol Dis. 39(3): 344-7.
  • Rosemary Siafakas A, et al. (2009) Growth arrest and DNA damage-45 alpha (GADD45alpha). Int J Biochem Cell Biol. 41(5): 986-9.
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