DPYS cDNA ORF Clone, Human, N-Myc tag General Information
Full length Clone DNA of Human dihydropyrimidinase with N terminal Myc tag.
Enhanced CMV promoter
Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
DPYS cDNA ORF Clone, Human, N-Myc tag Alternative Names
DHP cDNA ORF Clone, Human;DHPase cDNA ORF Clone, Human
DPYS Background Information
DPYS, also known as dihydropyrimidinase, belongs to the DHOase family, hydantoinase/dihydropyrimidinase subfamily. DPYS catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. It can catalyzes the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate. DPYS is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria.
Thomas HR. et al., 2008, Genomics. 18 (1): 25-35. Thomas HR. et al., 2008, Pharmacogenet Genomics. 17 (11): 973-87. Van Kuilenburg AB. et al., 2007, Mol Genet Metab. 91 (2): 157-64.