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Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Human TRDMT1 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_004412.4
Размер кДНК:1176bp
Описание кДНК:Full length Clone DNA of Homo sapiens tRNA aspartic acid methyltransferase 1 with N terminal Flag tag.
Синоним гена:DMNT2, DNMT2, PuMet, RNMT1, M.HsaIIP, TRDMT1
Виды:Human
переносчик:pCMV3-N-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка on other vectors
Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG11224-ACGRBS15400
Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG11224-ACRRBS15400
Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаHG11224-ANGRBS15400
Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаHG11224-ANRRBS15400
Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG11224-CFRBS13340
Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG11224-CHRBS13340
Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG11224-CMRBS13340
Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG11224-CYRBS13340
Человек DNMT2 / TRDMT1 Джин клон кДНК в вектор клонированияHG11224-MRBS5130
Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG11224-NFRBS13340
Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG11224-NHRBS13340
Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG11224-NMRBS13340
Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG11224-NYRBS13340
Человек DNMT2 / TRDMT1 Джин ORF экспрессии кДНК клона плазмидыHG11224-UTRBS13340
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Фон

DNMT2, also known as tRNA (cytosine-5-)-methyltransferase, DNA methyltransferase homolog HsaIIP, and TRDMT1, is a member of the DNA methyltransferase family of enzymes. DNMT2 enzymes have been widely conserved during evolution and contain all of the signature motifs of DNA (cytosine-5)-methyltransferases. It contains all 10 sequence motifs that are conserved among m(5)C MTases, including the consensus S:-adenosyl-L-methionine-binding motifs and the active site ProCys dipeptide, and its structure is very similar to prokaryotic DNA methyltransferases. DNMT2 has close homologs in plants, insects and Schizosaccharomyces pombe, but no related sequence can be found in the genomes of Saccharomyces cerevisiae or Caenorhabditis elegans. While the biological function of DNMT2 is not yet known, the strong binding to DNA suggests that DNMT2 may mark specific sequences in the genome by binding to DNA through the specific target-recognizing motif. However, the DNA methyltransferase activity of these proteins is comparatively weak and their biochemical and functional properties remain enigmatic. Recent evidence now shows that Dnmt2 has a novel tRNA methyltransferase activity, raising the possibility that the biological roles of these proteins might be broader than previously thought.

Ссылки
  • Dong A, et al. (2001) Structure of human DNMT2, an enigmatic DNA methyltransferase homolog that displays denaturant-resistant binding to DNA. Nucleic Acids Res. 29(2): 439-48.
  • Hermann A, et al. (2003) The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity. J Biol Chem. 278(34): 31717-21.
  • Jeltsch A, et al. (2006) Two substrates are better than one: dual specificities for Dnmt2 methyltransferases. Trends Biochem Sci. 31(6): 306-8.
  • Schaefer M, et al. (2010) Solving the Dnmt2 enigma. Chromosoma. 119(1): 35-40.
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    Каталог: HG11224-NF
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