After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!

Быстрый заказ

Text Size:AAA

Человек Cathepsin B/CTSB Джин ORF экспрессии кДНК клона плазмиды, C-HA Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Human CTSB Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001908.3
Размер кДНК:1020bp
Описание кДНК:Full length Clone DNA of Homo sapiens cathepsin B with C terminal HA tag.
Синоним гена:APPS; CPSB
Виды:Human
переносчик:pCMV3-C-HA
Plasmid:pCMV3-CTSB-HA
Участок рестрикции:KpnI(two restriction sites) + XbaI (6kb + 0.89kb + 0.18kb)
Последовательность меток:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Human CTSB Gene Plasmid Map
Human CTSB natural ORF mammalian expression plasmid, C-HA tag
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Человек Cathepsin B/CTSB Джин ORF экспрессии кДНК клона плазмиды, C-HA Метка on other vectors
Человек Cathepsin B/CTSB Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG10483-ACGRBS15396
Человек Cathepsin B/CTSB Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG10483-ACRRBS15396
Человек Cathepsin B/CTSB Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG10483-CFRBS13343
Человек Cathepsin B/CTSB Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG10483-CHRBS13343
Человек Cathepsin B/CTSB Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG10483-CMRBS13343
Человек Cathepsin B/CTSB Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG10483-CYRBS13343
Human CTSB Gene cDNA clone plasmidHG10483-MRBS3620
Человек Cathepsin B/CTSB Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG10483-NFRBS13343
Человек Cathepsin B/CTSB Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG10483-NHRBS13343
Человек Cathepsin B/CTSB Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG10483-NMRBS13343
Человек Cathepsin B/CTSB Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG10483-NYRBS13343
Человек Cathepsin B/CTSB Джин клон кДНК в вектор клонированияHG10483-URBS5132
Человек Cathepsin B/CTSB Джин ORF экспрессии кДНК клона плазмидыHG10483-UTRBS13343
 Узнайте больше о векторов экспрессии,
Product nameProduct name
Фон

Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.

Ссылки
  • Mai J, et al. (2000) Cell surface complex of cathepsin B/annexin II tetramer in malignant progression. Biochim Biophys Acta. 1477(1-2): 215-30.
  • Podgorski I, et al. (2003) Cathepsin B and its role(s) in cancer progression. Biochem Soc Symp. (70): 263-76.
  • Yan S, et al. (2003) Molecular regulation of human cathepsin B: implication in pathologies. Biol Chem. 384(6): 845-54.
  • Roshy S, et al. (2003) Pericellular cathepsin B and malignant progression. Cancer Metastasis Rev. 22(2-3): 271-86.
  •  

    Size / Price
    Каталог: HG10483-CY
    Цена по прейскуранту: 
    Цена:      (You Save: )
    Размер:
    Количество:+-
    НаличиеIn Stock
    Запрос по оптовому заказуДобавить в корзину
    Contact Us
    Изображения
    • Human CTSB natural ORF mammalian expression plasmid, C-HA tag
      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      Внимание: все продукты «ПРЕДНАЗНАЧЕНЫ ИСКЛЮЧИТЕЛЬНО ДЛЯ ИССЛЕДОВАНИЙ И НЕ ПРЕДНАЗНАЧЕНЫ ДЛЯ ДИАГНОСТИКИ ИЛИ ТЕРАПЕВТИЧЕСКОГО ЛЕЧЕНИЯ».