KK-LC-1 cDNA ORF Clone, Human, N-His tag

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KK-LC-1 cDNA ORF Clone, Human, N-His tag: General Information

Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
342 bp
Description
Full length Clone DNA of Human chromosome X open reading frame 61 with N terminal His tag.
Plasmid
Promoter
Enhanced CMV promoter
Tag Sequence
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

More Information About KK-LC-1

Entrez Gene ID

KK-LC-1 cDNA ORF Clone, Human, N-His tag: Alternative Names

CXorf61 cDNA ORF Clone, Human; KK-LC-1 cDNA ORF Clone, Human; KKLC1 cDNA ORF Clone, Human; RP3-452H17.2 cDNA ORF Clone, Human

KK-LC-1 Background Information

The CXorf61 sequence has first been identified by screening of a cDNA library derived from an allogeneic lung cancer cell line with an HLA-B*157-restricted cytotoxic T lymphocyte (CTL) clone established from a lung cancer patient. In a pilot screen of 2 normal tissues by RT-PCR (normal breast not included) testis tissue was the only one to express CXorf61 transcript. Later, two independent approaches, one mining of massive parallel signature sequencing (MPSS) data, the other providing a survey of CTAs in the Cancer Genome Atlas (TCGA) RNA-seq datasets revealed expression of CXorf61 transcripts in the basal-like subtype of breast cancer. The CXorf61 coding gene is located on chromosome Xq22 and consists of 113 amino acids.
Full Name
cancer/testis antigen 83
References
  • Paret C, Simon P, Vormbrock K, et al. CXorf61 is a target for T cell based immunotherapy of triple-negative breast cancer. Oncotarget. 2015;6(28):25356-25367.
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