After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!

Быстрый заказ

Text Size:AAA

Человек YKL-39/CHI3L2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Human CHI3L2 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:BC011460
Размер кДНК:1173bp
Описание кДНК:Full length Clone DNA of Homo sapiens chitinase 3-like 2 with C terminal Myc tag.
Синоним гена:YKL39, YKL-39, CHI3L2
Виды:Human
переносчик:pCMV3-C-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Человек YKL-39/CHI3L2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка on other vectors
Человек YKL-39/CHI3L2 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG12162-ACGRBS15400
Человек YKL-39/CHI3L2 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG12162-ACRRBS15400
Человек YKL-39/CHI3L2 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG12162-CFRBS13340
Человек YKL-39/CHI3L2 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG12162-CHRBS13340
Человек YKL-39/CHI3L2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG12162-CMRBS13340
Человек YKL-39/CHI3L2 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG12162-CYRBS13340
Человек YKL-39/CHI3L2 Джин клон кДНК в вектор клонированияHG12162-GRBS5130
Человек YKL-39/CHI3L2 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG12162-NFRBS13340
Человек YKL-39/CHI3L2 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG12162-NHRBS13340
Человек YKL-39/CHI3L2 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG12162-NMRBS13340
Человек YKL-39/CHI3L2 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG12162-NYRBS13340
Человек YKL-39/CHI3L2 Джин ORF экспрессии кДНК клона плазмидыHG12162-UTRBS13340
 Узнайте больше о векторов экспрессии,
Product nameProduct name
Фон

Chondrocyte protein 39 (YKL-39), also known as Chitinase 3-like 2 (CHI3L2), is a secretory protein of articular chondrocytes belonging to the glycosyl hydrolase 18 family. It highest expression is in chondrocytes, followed by synoviocytes, lung and heart. YKL-39/CHI3L2 is not detected in spleen, pancreas, and liver. YKL-39/CHI3L2 may also be expressed in developing brain and placenta. YKL-39/CHI3L2, a cartilage-related protein, is found to induce arthritis accompanied by pathologic changes in bone and cartilage. A better understanding of the immune response against cartilage-related components including YKL-39 may help to elucidate the pathological processes of arthritic disorders. Up regulation of YKL-39/CHI3L2 in osteoarthritic cartilage suggests that YKL-39/CHI3L2 may be a more accurate marker of chondrocyte activation than YKL-40, although it has yet to be established as a suitable marker in synovial fluid and serum. The decreased expression of YKL-40 by osteoarthritic chondrocytes is surprising as increased levels have been reported in rheumatoid and osteoarthritic synovial fluid, where it may derive from activated synovial cells or osteophytic tissue or by increased matrix destruction in the osteoarthritic joint. YKL-39 and YKL-40 are potentially interesting marker molecules for arthritic joint disease because they are abundantly expressed by both normal and osteoarthritic chondrocytes.

Ссылки
  • Sakata M, et al. (2002) YKL-39, a human cartilage-related protein, induces arthritis in mice. Clin Exp Rheumatol. 20(3): 343-50.
  • Areshkov PA, et al. (2010) Chitinase 3-like protein 2 (CHI3L2, YKL-39) activates phosphorylation of extracellular signal-regulated kinases ERK1/ERK2 in human embryonic kidney (HEK293) and human glioblastoma (U87 MG) cells. Tsitol Genet. 44(1): 3-9.
  • Steck E, et al. (2002) Enhanced expression of the human chitinase 3-like 2 gene (YKL-39) but not chitinase 3-like 1 gene (YKL-40) in osteoarthritic cartilage. Biochem Biophys Res Commun. 299(1): 109-15.
  • All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Внимание: все продукты «ПРЕДНАЗНАЧЕНЫ ИСКЛЮЧИТЕЛЬНО ДЛЯ ИССЛЕДОВАНИЙ И НЕ ПРЕДНАЗНАЧЕНЫ ДЛЯ ДИАГНОСТИКИ ИЛИ ТЕРАПЕВТИЧЕСКОГО ЛЕЧЕНИЯ».