CD5L qPCR Primer Pairs, Human General Information
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
SYBR® Green-based quantitative real-time PCR (qPCR).
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Lyophilized qPCR primer mix is shipped at ambiente temperatura
The lyophilized product is stable for one year from date of receipt when stored at -20℃.
The suspended product is stable for six months from date of receipt when stored at -20℃.
***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***
Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
CD5L qPCR Primer Pairs, Human Alternative Names
AIM qPCR Primer Pairs, Human;API6 qPCR Primer Pairs, Human;PRO229 qPCR Primer Pairs, Human;SP-ALPHA qPCR Primer Pairs, Human;Spalpha qPCR Primer Pairs, Human
CD5L Background Information
CD5L, also known as CD5 antigen-like, is a soluble protein belonging to group B of the scavenger receptor cysteine-rich (SRCR) superfamily and contains three SRCR domains. It is a secreted glycoprotein and expressed by macrophages presentin lymphoid tissues (spleen, lymph node, thymus, and bone marrow). It binds to myelomonocytic and lymphoid cells and may play an important role in the regulation of the innate and adaptive immune systems. CD5L functions as a pattern recognition molecule by binding both lipoteichoic acid (LTA) on Gram positive and lipopolysaccharide (LPS) on Gram negative bacteria. and the SRCR domain 1 of CD5L retains both the LPS and LTA binding activities. In addtion, it is revealed that CD5L seems to play a role as an inhibitor of apoptosis.
Resnick, D. et al., 1994, Trends Biochem. Sci. 19: 5-8. Gebe, J. A. et al., 1997, J. Biol. Chem. 272 (10): 6151–6158. Sarrias, M.R. et al., 2004, Crit. Rev. Immunol. 24: 1-37. Mukhopadhyay, S. and Gordon, S., 2004, Immunobiology 209: 39-49. Sarrias, M. R. et al.,2005, J. Biol. Chem. 280 (42): 35391–35398.