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Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмиды, C-HA Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Human CCL21 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_002989.2
Размер кДНК:405bp
Описание кДНК:Full length Clone DNA of Homo sapiens chemokine (C-C motif) ligand 21 with C terminal HA tag.
Синоним гена:ECL, SLC, CKb9, TCA4, 6Ckine, SCYA21, MGC34555, CCL21
Виды:Human
переносчик:pCMV3-C-HA
Plasmid:
Участок рестрикции:
Последовательность меток:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмиды, C-HA Метка on other vectors
Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG10477-ACGRBS15400
Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG10477-ACRRBS15400
Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG10477-CFRBS13340
Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG10477-CHRBS13340
Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG10477-CMRBS13340
Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG10477-CYRBS13340
Человек CCL21/6Ckine Джин клон кДНК в вектор клонированияHG10477-MRBS5130
Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG10477-M-FRBS13340
Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG10477-NFRBS13340
Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG10477-NHRBS13340
Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG10477-NMRBS13340
Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG10477-NYRBS13340
Человек CCL21/6Ckine Джин ORF экспрессии кДНК клона плазмидыHG10477-UTRBS13340
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Фон

Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 21(CCL21), also known as 6Ckine, exodus-2, and secondary lymphoid-tissue chemokine(SLC), is a small cytokine belonging to the C-C chemokine family. CCL21 takes its name 6Ckine for its consititutively six conserved cysteine residues but not four cysteines typical to chemokines. CCL21 has function in ininducing vigorous calcium migrations and chemotactic responses.

Ссылки
  • Laing KJ, et al. (2004) Chemokines. Developmental and comparative immunology. 28(5): 443-60.
  • Cocchi F, et al. (1995) Identification of RANTES, MIP-1a, and MIP-1b as the major HIV-suppressive factor produced by CD8+ T cells. Science. 270 (5243): 1811-5.
  • Yoshida R, et al. (1998) Secondary lymphoid-tissue chemokine is a functional ligand for the CC chemokine receptor CCR7. The journal of biological chemistry. 273: 7118-22.
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