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Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, C-His Метка

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    Человек CA13 Информация о продукте «Клон cDNA»
    Gene_bank_ref_id:NM_198584.2
    Размер кДНК:789bp
    Описание кДНК:Full length Clone DNA of Homo sapiens carbonic anhydrase XIII with C terminal His tag.
    Синоним гена:CAXIII, FLJ37995, MGC59868, CA13
    Виды:Human
    переносчик:pCMV3-C-His
    Plasmid:
    Участок рестрикции:
    Последовательность меток:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
    Описание последовательности:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with CA13 qPCR primers for gene expression analysis, HP100489 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Склад:The lyophilized plasmid can be stored at room temperature for three months.
    His Tag Info

    A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

    Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, C-His Метка on other vectors
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG10461-ACGRBS15400
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG10461-ACRRBS15400
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаHG10461-ANGRBS15400
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаHG10461-ANRRBS15400
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG10461-CFRBS13340
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG10461-CHRBS13340
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG10461-CMRBS13340
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG10461-CYRBS13340
    Человек Carbonic Anhydrase XIII/CA13 Джин клон кДНК в вектор клонированияHG10461-MRBS5130
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG10461-M-FRBS13340
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG10461-NFRBS13340
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG10461-NHRBS13340
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG10461-NMRBS13340
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG10461-NYRBS13340
    Человек Carbonic Anhydrase XIII/CA13 Джин ORF экспрессии кДНК клона плазмидыHG10461-UTRBS13340
     Узнайте больше о векторов экспрессии,
    Product nameProduct name
    Фон

    The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. The CAXIII is a member of the CA family, which owns a globular molecule with high structural similarity to cytosolic isozymes, CAI, II, and III. Recombinant mouse CAXIII showed catalytic activity similar to those of mitochondrial CAV and cytosolic CAI. In human tissues, CAXIII expression was identified in the thymus, small intestine, spleen, prostate, ovary, colon, and testis. In mouse, positive tissues included the spleen, lung, kidney, heart, brain, skeletal muscle, and testis. In conclusion, the predicted amino acid sequence, structural model, distribution, and activity data suggest that CAXIII represents a novel enzyme, which may play important physiological roles in several organs.

    Ссылки
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
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    Каталог: HG10461-CH
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