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Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Human BTC Информация о продукте «Клон cDNA»
Gene_bank_ref_id:BC011618
Размер кДНК:537bp
Описание кДНК:Full length Clone DNA of Homo sapiens betacellulin with C terminal Myc tag.
Синоним гена:BTC
Виды:Human
переносчик:pCMV3-C-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка on other vectors
Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG12192-ACGRBS15400
Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG12192-ACRRBS15400
Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG12192-CFRBS13340
Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG12192-CHRBS13340
Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG12192-CMRBS13340
Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG12192-CYRBS13340
Человек Betacellulin / BTC Джин клон кДНК в вектор клонированияHG12192-GRBS5130
Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG12192-G-FRBS13340
Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG12192-NFRBS13340
Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG12192-NHRBS13340
Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG12192-NMRBS13340
Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG12192-NYRBS13340
Человек Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмидыHG12192-UTRBS13340
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Фон

Betacellulin(BTC) is a member of the epidermal growth factor (EGF) family. These soluble proteins are ligands for one or more of the four receptor tyrosine kinases encoded by the ErbB gene family (ErbB-1/epidermal growth factor receptor (EGFR), neu/ErbB-2/HER2, ErbB-3/HER3 and ErbB-4/HER4). Betacellulin is a 32-kilodalton glycoprotein that appears to be processed from a larger transmembrane precursor by proteolytic cleavage. This protein is a ligand for the EGF receptor. BTC is a polymer of about 62-111 amino acid residues. Secondary Structure: 6% helical (1 helices; 3 residues)36% beta sheet (5 strands; 18 residues). BTC was originally identified as a growth-promoting factor in mouse pancreatic β-cell carcinoma cell line and has since been identified in humans. It plays a role in the growth and development of the neonate and/or mammary gland function. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Ссылки
  • Shing Y, et al. (1993) Betacellulin: a mitogen from pancreatic beta cell tumors. Science . 259(5101): 1604-7.
  • Riese DJ, et al. (1996) Betacellulin activates the epidermal growth factor receptor and erbB-4, and induces cellular response patterns distinct from those stimulated by epidermal growth factor or neuregulin-beta. Oncogene. 12(2): 345-53.
  • Bastian SE, et al. (2001) Measurement of betacellulin levels in bovine serum, colostrum and milk. J Endocrinol . 168: 203-12.
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    Каталог: HG12192-CM
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