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Человек BIK Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Human BIK Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001197.3
Размер кДНК:483bp
Описание кДНК:Full length Clone DNA of Homo sapiens BCL2-interacting killer (apoptosis-inducing) with C terminal Myc tag.
Синоним гена:BP4, NBK, BIP1, BIK
Виды:Human
переносчик:pCMV3-C-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Человек BIK Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка on other vectors
Человек BIK Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG11239-ACGRBS15400
Человек BIK Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG11239-ACRRBS15400
Человек BIK Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаHG11239-ANGRBS15400
Человек BIK Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаHG11239-ANRRBS15400
Человек BIK Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG11239-CFRBS13340
Человек BIK Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG11239-CHRBS13340
Человек BIK Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG11239-CMRBS13340
Человек BIK Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG11239-CYRBS13340
Человек BIK Джин клон кДНК в вектор клонированияHG11239-MRBS5130
Человек BIK Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG11239-NFRBS13340
Человек BIK Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG11239-NHRBS13340
Человек BIK Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG11239-NMRBS13340
Человек BIK Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG11239-NYRBS13340
Человек BIK Джин ORF экспрессии кДНК клона плазмидыHG11239-UTRBS13340
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Фон

The BIN1 protein, which is encoded by the BIN1 gene, belongs to Myc-interacting protein family which is one of the nucleocytoplasmic adaptor proteins. The BIN1 protein can interact with the functionally critically Myc-box region at the N-terminal of the Myc oncoprotein, with a feature of tumor suppressor. Myc family proteins promote proliferation, growth, and apoptosis and, when deregulated, are profoundly involved in the genesis of an extraordinarily wide range of cancers. Although BIN1 is expressed in many normal cells, its levels were greatly reduced or undetectable in 14/27 carcinoma cell lines and 3/6 primary breast tumours. Deficits were functionally Although BIN1 is expressed in many normal cells, its levels were greatly reduced or undetectable in 14/27 carcinoma cell lines and 3/6 primary breast tumours. Deficits were functionally significant because ectopic expression of BIN1 inhibited the growth of tumour cells lacking endogenous message. We conclude that BIN1 is an MYC-interacting protein with features of a tumour suppressor.

Ссылки
  • Boyd J.M., et al.,(1995), Bik, a novel death-inducing protein shares a distinct sequence motif with Bcl-2 family proteins and interacts with viral and cellular survival-promoting proteins. Oncogene 11:1921-1928.
  • Han J., et al., (1996), Induction of apoptosis by human Nbk/Bik, a BH3-containing protein that interacts with E1B 19K.Mol. Cell. Biol. 16:5857-5864.
  • Hegde R., et al.,(1998), Blk, a BH3-containing mouse protein that interacts with Bcl-2 and Bcl-xL, is a potent death agonist.J. Biol. Chem. 273:7783-7786.
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