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Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Human APOH Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_000042.2
Размер кДНК:1038bp
Описание кДНК:Full length Clone DNA of Homo sapiens apolipoprotein H (beta-2-glycoprotein I) with N terminal Flag tag.
Синоним гена:BG, B2G1, APOH
Виды:Human
переносчик:pCMV3-SP-N-FLAG
Plasmid:pCMV3-Flag-APOH
Участок рестрикции:KpnI + XbaI (6kb + 1.07kb)
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Human APOH Gene Plasmid Map
Human Apolipoprotein H / APOH natural ORF mammalian expression plasmid, N-Flag tag
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка on other vectors
Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG11221-ACGRBS15396
Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG11221-ACRRBS15400
Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG11221-CFRBS13343
Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG11221-CHRBS13343
Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG11221-CMRBS13343
Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG11221-CYRBS13343
Человек Apolipoprotein H/APOH Джин клон кДНК в вектор клонированияHG11221-MRBS5132
Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмидыHG11221-M-NRBS13343
Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG11221-NFRBS13343
Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG11221-NHRBS13343
Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG11221-NMRBS13343
Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG11221-NYRBS13343
Человек Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмидыHG11221-UTRBS13343
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Фон

Apolipoprotein H (APOH), also known as Beta-2-glycoprotein 1, Activated protein C-binding protein, B2GPI, and B2G1, is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. It is an essential cofactor for the binding of certain antiphospholipid antibodies (APA) to anionic phospholipid. APOH binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. APOH may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. APOH appears to completely inhibit serotonin release by the platelets and prevents subsequent waves of the ADP-induced aggregation. The activity of APOH appears to involve the binding of agglutenating, negatively charged compounds, and inhibits agglutenation by the contact activation of the intrinsic blood coagulation pathway. APOH causes a reduction of the prothrombinase binding sites on platelets and reduces the activation caused by collagen when thrombin is present at physiological serum concentrations of APOH suggesting a regulatory role of APOH in coagulation. APOH plasma concentrations are strongly associated to metabolic syndrome alterations and vascular disease in type 2 diabetic and could be considered as a clinical marker of cardiovascular risk. APOH is found on several classes of lipoproteins, and is involved in the activation of lipoprotein lipase in lipid metabolism. This single-chain glycoprotein also has been implicated in several physiologic pathways including coagulation and the production of hypertension, which are related to the pathogenesis of primary cerebral hemorrhage (PICH).

Ссылки
  • Kamboh MI, et al. (1998) Genetics of apolipoprotein H (beta2-glycoprotein I) and anionic phospholipid binding. Lupus. 7 Suppl 2: S10-3.
  • Singh P, et al. (2002) Genetics of apolipoprotein H (beta2-glycoprotein I) polymorphism in India. Ann Hum Biol. 29(3): 247-55.
  • Xia J, et al. (2004) Apolipoprotein H gene polymorphisms and risk of primary cerebral hemorrhage in a Chinese population. Cerebrovasc Dis. 17(2-3): 197-203.
  • Chen Q, et al. (2006) Complete DNA sequence variation in the apolipoprotein H (beta-glycoprotein I) gene and identification of informative SNPs. Ann Hum Genet. 70(Pt 1): 1-11.
  • Leduc MS, et al. (2008) Comprehensive evaluation of apolipoprotein H gene (APOH) variation identifies novel associations with measures of lipid metabolism in GENOA. J Lipid Res. 49(12): 2648-56.
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    Каталог: HG11221-NF
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