The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
ASF1A cDNA ORF Clone, Human, N-DDK (Flag®) tag: Alternative Names
CGI-98 cDNA ORF Clone, Human; CIA cDNA ORF Clone, Human; HSPC146 cDNA ORF Clone, Human
ASF1A Background Information
ASF1A has been reported to be dysregulated in multiple tumors, USP52 is a bona fide ubiquitin-specific protease, and USP52 promotes ASF1A deubiquitination and stabilization. USP52-promoted ASF1A stabilization facilitates chromatin assembly and favors cell cycle progression. USP52-promoted ASF1A stabilization results in growth arrest of breast cancer cells and sensitizes these cells to DNA damage. Histone chaperone, anti-silencing function-1 A (ASF1A), is crucial for post-repair H3K56Ac restoration, which in turn, is needed for the dephosphorylation of gamma-H2AX and cellular recovery from checkpoint arrest.