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Человек Angiotensinogen/SerpinA8 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Human AGT Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_000029.3
Размер кДНК:1446bp
Описание кДНК:Full length Clone DNA of Homo sapiens angiotensinogen (serpin peptidase inhibitor, clade A, member 8) with C terminal Flag tag.
Синоним гена:ANHU, FLJ92595, FLJ97926, SERPINA8
Виды:Human
переносчик:pCMV3-C-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Человек Angiotensinogen/SerpinA8 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Человек Angiotensinogen/SerpinA8 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG10994-ACGRBS15400
Человек Angiotensinogen/SerpinA8 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG10994-ACRRBS15400
Человек Angiotensinogen/SerpinA8 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG10994-CFRBS13340
Человек Angiotensinogen/SerpinA8 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG10994-CHRBS13340
Человек Angiotensinogen/SerpinA8 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG10994-CMRBS13340
Человек Angiotensinogen/SerpinA8 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG10994-CYRBS13340
Человек Angiotensinogen/SerpinA8 Джин клон кДНК в вектор клонированияHG10994-MRBS5130
Человек Angiotensinogen/SerpinA8 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG10994-NFRBS13340
Человек Angiotensinogen/SerpinA8 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG10994-NHRBS13340
Человек Angiotensinogen/SerpinA8 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG10994-NMRBS13340
Человек Angiotensinogen/SerpinA8 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG10994-NYRBS13340
Человек Angiotensinogen/SerpinA8 Джин ORF экспрессии кДНК клона плазмидыHG10994-UTRBS13340
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Фон

Angiotensinogen, also known as AGT and SerpinA8, is a member of the serpin family. It is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. Angiotensinogen can be schematically considered to consist of a combination of an angiotensin I (Ang I) function, located at the N-terminal end, and the presence of a serpin (serine protease inhibitor) structure at the opposite end. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD). Several serpins (antithrombin, maspin, pigment epithelial-derived factor, and kallistatin) have been recently shown to exert an antiangiogenic activity, suggesting a common mechanism of endothelial cell proliferation and migration. Angiotensinogen/AGT and its renin-cleaved product, des(Ang I)AGT, are also angiogenesis inhibitors, both in vitro and in vivo at concentrations within the range of those observed in plasma. The Angiotensinogen products, that is angiotensin II and possibly angiotensin II-related products, have been found to act locally in modulating adipose tissue growth in an autocrine/paracrine manner. The transient or chronic overexpression of angiotensinogen in adipose tissue favors lipogenesis in adipocytes and leads to a 'vicious' circle whereby adipose tissue development is further increased.

Ссылки
  • Ailhaud G, et al. (2002) Angiotensinogen, adipocyte differentiation and fat mass enlargement. Curr Opin Clin Nutr Metab Care. 5(4): 385-9.
  • Corvol P, et al. (2003) Inhibition of angiogenesis: a new function for angiotensinogen and des(angiotensin I)angiotensinogen. Curr Hypertens Rep. 5(2): 149-54.
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    Каталог: HG10994-CF
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