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Резус-фактор HSPA8/HSC70 Джин клон кДНК в вектор клонирования

ПаспортОбзорыСвязанные продуктыПротоколы
Cynomolgus HSPA8 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001261657.2
Размер кДНК:1941bp
Описание кДНК:Full length Clone DNA of Macaca mulatta (Rhesus monkey) heat shock 70kDa protein 8.
Синоним гена:HSPA8
Виды:Rhesus
переносчик:pGEM-T Vector
Plasmid:pGEM-cynoHSPA8
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 626/T/C(I209T), 1019C/A(S340Y); 1101G/A, 1138T/C not causing the amino acid variation. Please check the sequence information before order.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Резус-фактор HSPA8/HSC70 Джин клон кДНК в вектор клонирования on other vectors
Резус-фактор HSPA8/HSC70 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаCG90471-ACGRBS16760
Резус-фактор HSPA8/HSC70 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаCG90471-ACRRBS16760
Резус-фактор HSPA8/HSC70 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаCG90471-CFRBS14710
Резус-фактор HSPA8/HSC70 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаCG90471-CHRBS14710
Резус-фактор HSPA8/HSC70 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаCG90471-CMRBS14710
Резус-фактор HSPA8/HSC70 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаCG90471-CYRBS14710
Резус-фактор HSPA8/HSC70 Джин клон кДНК в вектор клонированияCG90471-GRBS5130
Резус-фактор HSPA8/HSC70 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаCG90471-NFRBS14710
Резус-фактор HSPA8/HSC70 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаCG90471-NHRBS14710
Резус-фактор HSPA8/HSC70 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаCG90471-NMRBS14710
Резус-фактор HSPA8/HSC70 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаCG90471-NYRBS14710
Резус-фактор HSPA8/HSC70 Джин ORF экспрессии кДНК клона плазмидыCG90471-UTRBS14710
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Фон

HSPA8, also known as HSC70, is a member of the heat shock protein family due to homology with other heat shock proteins. The heat shock protein 70 family is comprised by both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 belongs to the heat-shock cognate subgroup. Members of the human heat-shock protein multigene family have several highly conserved proteins with structural and functional properties in common, but vary in the extent of their inducibility in response to metabolic stress. HSPA8 is constitutively expressed and performs functions related to normal cellular processes. This protein binds to nascent polypeptides to facilitate correct protein folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. HSPA8 acts as a repressor of transcriptional activation. It inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones. It also is a ATPase that works with auxilin to remove clathrin coated vesicles. In neurons, synaptojanin is also an important protein involved in vesicle uncoating.

Ссылки
  • Santacruz H, et al. (1997) Molecular characterization of a heat shock cognate cDNA of zebrafish, hsc70, and developmental expression of the corresponding transcripts. Dev Genet. 21:223-33.
  • Harhay G P, et al. (2005) Characterization of 954 bovine full-CDS cDNA sequences. BMC Genomics. 6: 166.
  • Goldfarb S, et al. (2006) Differential effects of Hsc70 and Hsp70 on the intracellular trafficking and functional expression of epithelial sodium channels. Proc Natl Acad Sci. 103(15):5817-22.
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    Каталог: CG90471-G
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