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Человек RACK1/GNB2L1 Джин клон кДНК в вектор клонирования

ПаспортОбзорыСвязанные продуктыПротоколы
Human GNB2L1 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:BC019362.1
Размер кДНК:954bp
Описание кДНК:Full length Clone DNA of Homo sapiens guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1.
Синоним гена:H12.3, HLC-7, PIG21, RACK1, Gnb2-rs1
Виды:Human
переносчик:pGEM-T Vector
Plasmid:pGEM-hGNB2L1
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Человек RACK1/GNB2L1 Джин клон кДНК в вектор клонирования on other vectors
Человек RACK1/GNB2L1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG16196-ACGRBS15400
Человек RACK1/GNB2L1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG16196-ACRRBS15400
Человек RACK1/GNB2L1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG16196-CFRBS13340
Человек RACK1/GNB2L1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG16196-CHRBS13340
Человек RACK1/GNB2L1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG16196-CMRBS13340
Человек RACK1/GNB2L1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG16196-CYRBS13340
Человек RACK1/GNB2L1 Джин клон кДНК в вектор клонированияHG16196-GRBS5130
Человек RACK1/GNB2L1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG16196-NFRBS13340
Человек RACK1/GNB2L1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG16196-NHRBS13340
Человек RACK1/GNB2L1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG16196-NMRBS13340
Человек RACK1/GNB2L1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG16196-NYRBS13340
Человек RACK1/GNB2L1 Джин ORF экспрессии кДНК клона плазмидыHG16196-UTRBS13340
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Фон

RACK1 (guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1), also known as GNB2L1, contains 7 WD repeats and belongs to the WD repeat G protein beta family. In the liver, RACK1 is expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. It is up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue. RACK1 is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. It interacts with a wide variety of proteins and plays a role in many cellular processes. GNB2L1 binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. RACK1 may recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. It inhibits the activity of SRC kinases including SRC, LCK and YES1. RACK1 also inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. It enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer.

Ссылки
  • Jannot G, et al.. (2011) The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans. EMBO Rep. 12(6):581-6.
  • Wang F, et al. (2011) RACK1 regulates VEGF/Flt1-mediated cell migration via activation of a PI3K/Akt pathway. J Biol Chem. 286(11):9097-106.
  • Cao XX, et al. (2011) RACK1 promotes breast carcinoma migration/metastasis via activation of the RhoA/Rho kinase pathway. Breast Cancer Res Treat. 126(3):555-63.
  • Myklebust LM, et al. (2011) Receptor for activated protein C kinase 1 (RACK1) is overexpressed in papillary thyroid carcinoma. Thyroid. 21(11):1217-25.
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    Каталог: HG16196-G
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