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Человек GMFB Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Human GMFB Информация о продукте «Клон cDNA»
Gene_bank_ref_id:BC005359
Размер кДНК:465bp
Описание кДНК:Full length Clone DNA of Homo sapiens glia maturation factor, beta with Flag tag.
Синоним гена:GMF, GMFB
Виды:Human
переносчик:pCMV/hygro-FLAG
Plasmid:pCMV-GMFB-flag
Участок рестрикции:KpnI + XhoI (5.5kb + 0.5kb)
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Human GMFB Gene Plasmid Map
Human GMFB Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged
pCMV/hygro-FLAG Vector Information
 
Vector Name pCMV/hygro-FLAG
Vector Size 5681bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag FLAG
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

Schematic of pCMV/hygro-FLAG Multiple Cloning Sites

FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Человек GMFB Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Человек GMFB Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG13244-ACGRBS15400
Человек GMFB Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG13244-ACRRBS15400
Человек GMFB Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG13244-CFRBS13340
Человек GMFB Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG13244-CHRBS13340
Человек GMFB Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG13244-CMRBS13340
Человек GMFB Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG13244-CYRBS13340
Человек GMFB Джин клон кДНК в вектор клонированияHG13244-GRBS5130
Человек GMFB Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG13244-NFRBS13340
Человек GMFB Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG13244-NHRBS13340
Человек GMFB Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG13244-NMRBS13340
Человек GMFB Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG13244-NYRBS13340
Человек GMFB Джин ORF экспрессии кДНК клона плазмидыHG13244-UTRBS13340
 Узнайте больше о векторов экспрессии,
Product nameProduct name
Фон

GMFB is a nerve growth factor which belongs to the actin-binding proteins ADF family, GMF subfamily. GMFB is involved in nervous system development, angiogenesis and immune function. It is especially crucial for the nervous system. GMFB causes brain cell differentiation, stimulates neural regeneration and inhibits tumor cell proliferation. It contains 1 ADF-H domain and is phosphorylated after phorbol ester stimulation. GMFB overexpression in astrocytes results in the increase of BDNF production. GMFB expression is increased by exercise, thus BDNF is important for exercise-induction of BDNF.

Ссылки
  • Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40.
  • Zaheer S, et al. (2011) Augmented expression of glia maturation factor in Alzheimer's disease. Neuroscience. 194:227-33.
  • Danielsen JM, et al. (2011) Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level. Mol Cell Proteomics. 10(3):M110.003590.
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    Каталог: HG13244-G-F
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