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Яванский макак GAPDH Джин клон кДНК в вектор клонирования

ПаспортОбзорыСвязанные продуктыПротоколы
Cynomolgus GAPDH Информация о продукте «Клон cDNA»
Gene_bank_ref_id:XM_005569912.1
Размер кДНК:1008bp
Описание кДНК:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) glyceraldehyde-3-phosphate dehydrogenase.
Синоним гена:GAPDH
Виды:Cynomolgus
переносчик:pGEM-T Vector
Plasmid:pGEM-cynoGAPDH
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 282T/C, 300T/C, 912C/T not causing the amino acid variation. Please check the sequence information before order.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Яванский макак GAPDH Джин клон кДНК в вектор клонирования on other vectors
Яванский макак GAPDH Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаCG90448-ACGRBS15400
Яванский макак GAPDH Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаCG90448-ACRRBS15400
Яванский макак GAPDH Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаCG90448-ANGRBS15400
Яванский макак GAPDH Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаCG90448-ANRRBS15400
Яванский макак GAPDH Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаCG90448-CFRBS13340
Яванский макак GAPDH Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаCG90448-CHRBS13340
Яванский макак GAPDH Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаCG90448-CMRBS13340
Яванский макак GAPDH Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаCG90448-CYRBS13340
Яванский макак GAPDH Джин клон кДНК в вектор клонированияCG90448-GRBS5130
Яванский макак GAPDH Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаCG90448-NFRBS13340
Яванский макак GAPDH Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаCG90448-NHRBS13340
Яванский макак GAPDH Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаCG90448-NMRBS13340
Яванский макак GAPDH Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаCG90448-NYRBS13340
Яванский макак GAPDH Джин ORF экспрессии кДНК клона плазмидыCG90448-UTRBS13340
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Фон

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

Ссылки
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
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