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Крыса Coagulation Factor II/F2 Джин клон кДНК в вектор клонирования

ПаспортОбзорыСвязанные продуктыПротоколы
Rat F2 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_022924.2
Размер кДНК:1854bp
Описание кДНК:Full length Clone DNA of Rattus norvegicus coagulation factor II.
Синоним гена:F2
Виды:Rat
переносчик:pUC19 Vector
Plasmid:cpUC19-ratF2
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 492C>T, 1467C>T and 1527T>C not causing the amino acid variation; 5C>T (S2L).
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Rat F2 Gene Plasmid Map
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Крыса Coagulation Factor II/F2 Джин клон кДНК в вектор клонирования on other vectors
Крыса Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаRG80722-ACGRBS16760
Крыса Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаRG80722-ACRRBS16760
Крыса Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаRG80722-CFRBS14710
Крыса Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаRG80722-CHRBS14710
Крыса Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаRG80722-CMRBS14710
Крыса Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаRG80722-CYRBS14710
Крыса Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаRG80722-NFRBS14710
Крыса Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаRG80722-NHRBS14710
Крыса Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаRG80722-NMRBS14710
Крыса Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаRG80722-NYRBS14710
Крыса Coagulation Factor II/F2 Джин клон кДНК в вектор клонированияRG80722-URBS5130
Крыса Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмидыRG80722-UTRBS14710
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Фон

Coagulation Factor II Protein (FII, F2 Protein or Prothrombin) is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. Coagulation Factor II Protein (FII, F2 Protein) also plays a role in maintaining vascular integrity during development and postnatal life. Prothrombin / Coagulation Factor II is activated on the surface of a phospholipid membrane that binds the amino end of prothrombin / Coagulation Factor II and factor Va and Xa in Ca-dependent interactions; factor Xa removes the activation peptide and cleaves the remaining part into light and heavy chains. The activation process starts slowly because factor V itself has to be activated by the initial, small amounts of thrombin. Prothrombin / Coagulation Factor II is expressed by the liver and secreted in plasma. Defects in prothrombin / Coagulation Factor II are the cause of factor II deficiency (FA2D). It is very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Defects in Coagulation Factor II are also a cause of susceptibility to thrombosis. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.

Ссылки
  • Danneberg J, et al. (1998) Reliable genotyping of the G-20210-A mutation of Coagulation Factor II (prothrombin). Clin Chem. 44(2): 349-51.
  • Redondo M, et al. (1999) Coagulation Factor s II, V, VII, and X, prothrombin gene 20210GA transition, and factor V Leiden in coronary artery disease: high factor V clotting activity is an independent risk factor for myocardial infarction. Arterioscler Thromb Vasc Biol. 19(4): 1020-5.
  • Miletich JP, et al. (1980) The synthesis of sulfated dextran beads for isolation of human plasma Coagulation Factor s II, IX, and X. Anal Biochem. 105(2): 304-10.
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    Каталог: RG80722-U
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