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Резус-фактор P-Selectin/CD62P/SELP Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Cynomolgus SELP Информация о продукте «Клон cDNA»
Gene_bank_ref_id:XM_001094728.2
Размер кДНК:2493bp
Описание кДНК:Full length Clone DNA of Macaca mulatta (Rhesus monkey) selectin P (granule membrane protein 140kDa, antigen CD62) with C terminal Flag tag.
Синоним гена:SELP
Виды:Rhesus
переносчик:pCMV3-C-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Резус-фактор P-Selectin/CD62P/SELP Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Резус-фактор P-Selectin/CD62P/SELP Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаCG90170-ACGRBS16760
Резус-фактор P-Selectin/CD62P/SELP Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаCG90170-ACRRBS16760
Резус-фактор P-Selectin/CD62P/SELP Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаCG90170-CFRBS14710
Резус-фактор P-Selectin/CD62P/SELP Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаCG90170-CHRBS14710
Резус-фактор P-Selectin/CD62P/SELP Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаCG90170-CMRBS14710
Резус-фактор P-Selectin/CD62P/SELP Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаCG90170-CYRBS14710
Резус-фактор P-Selectin/CD62P/SELP Джин клон кДНК в вектор клонированияCG90170-GRBS5130
Резус-фактор P-Selectin/CD62P/SELP Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаCG90170-NFRBS14710
Резус-фактор P-Selectin/CD62P/SELP Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаCG90170-NHRBS14710
Резус-фактор P-Selectin/CD62P/SELP Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаCG90170-NMRBS14710
Резус-фактор P-Selectin/CD62P/SELP Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаCG90170-NYRBS14710
Резус-фактор P-Selectin/CD62P/SELP Джин ORF экспрессии кДНК клона плазмидыCG90170-UTRBS14710
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Фон

P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.

Ссылки
  • Johnson-Tidey RR, et al. (1994) Increase in the adhesion molecule P-selectin in endothelium overlying atherosclerotic plaques. Coexpression with intercellular adhesion molecule-1. Am J Pathol. 144(5):952-61.
  • Walcheck B, et al. (1996) Neutrophil-neutrophil interactions under hydrodynamic shear stress involve L-selectin and PSGL-1. A mechanism that amplifies initial leukocyte accumulation of P-selectin in vitro. J Clin Invest. 98(5):1081-7.
  • Foreman KE, et al. (1994) C5a-induced expression of P-selectin in endothelial cells. J Clin Invest. 94(3):1147-55.
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    Каталог: CG90170-CF
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