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Резус-фактор CD10/Neprilysin Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Cynomolgus MME Информация о продукте «Клон cDNA»
Gene_bank_ref_id:XM_002802963.1
Размер кДНК:2253bp
Описание кДНК:Full length Clone DNA of Macaca mulatta (Rhesus monkey) membrane metallo-endopeptidase with C terminal Flag tag.
Синоним гена:MME
Виды:Rhesus
переносчик:pCMV3-C-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Резус-фактор CD10/Neprilysin Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Резус-фактор CD10/Neprilysin Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаCG90177-ACGRBS16760
Резус-фактор CD10/Neprilysin Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаCG90177-ACRRBS16760
Резус-фактор CD10/Neprilysin Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаCG90177-CFRBS14710
Резус-фактор CD10/Neprilysin Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаCG90177-CHRBS14710
Резус-фактор CD10/Neprilysin Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаCG90177-CMRBS14710
Резус-фактор CD10/Neprilysin Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаCG90177-CYRBS14710
Резус-фактор CD10/Neprilysin Джин клон кДНК в вектор клонированияCG90177-GRBS5130
Резус-фактор CD10/Neprilysin Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаCG90177-NFRBS14710
Резус-фактор CD10/Neprilysin Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаCG90177-NHRBS14710
Резус-фактор CD10/Neprilysin Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаCG90177-NMRBS14710
Резус-фактор CD10/Neprilysin Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаCG90177-NYRBS14710
Резус-фактор CD10/Neprilysin Джин ORF экспрессии кДНК клона плазмидыCG90177-UTRBS14710
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Product nameProduct name
Фон

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had function to degrade a number of small secreted peptides such as the amyloid beta peptide. It exist as a membrane-bound protein and have high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes.

Ссылки
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12
  • Dogan, et al. (2000) CD10 and BCL-6 Expression in Paraffin Sections of Normal Lymphoid Tissue and B-Cell Lymphomas. American Journal of Surgical Pathology. 24(6): 846-52.
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    Каталог: CG90177-CF
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    Количество:+-
    Наличие2-3 weeks
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