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Резус-фактор Hemojuvelin / HFE2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Cynomolgus HFE2 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:XM_001093102.2
Размер кДНК:1284bp
Описание кДНК:Full length Clone DNA of Macaca mulatta (Rhesus monkey) hemochromatosis type 2 (juvenile) with C terminal Myc tag.
Синоним гена:HFE2
Виды:Rhesus
переносчик:pCMV3-C-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Резус-фактор Hemojuvelin / HFE2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка on other vectors
Резус-фактор Hemojuvelin / HFE2 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаCG90061-ACGRBS15400
Резус-фактор Hemojuvelin / HFE2 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаCG90061-ACRRBS15400
Резус-фактор Hemojuvelin / HFE2 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаCG90061-CFRBS13340
Резус-фактор Hemojuvelin / HFE2 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаCG90061-CHRBS13340
Резус-фактор Hemojuvelin / HFE2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаCG90061-CMRBS13340
Резус-фактор Hemojuvelin / HFE2 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаCG90061-CYRBS13340
Резус-фактор Hemojuvelin / HFE2 Джин клон кДНК в вектор клонированияCG90061-GRBS5130
Резус-фактор Hemojuvelin / HFE2 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаCG90061-NFRBS13340
Резус-фактор Hemojuvelin / HFE2 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаCG90061-NHRBS13340
Резус-фактор Hemojuvelin / HFE2 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаCG90061-NMRBS13340
Резус-фактор Hemojuvelin / HFE2 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаCG90061-NYRBS13340
Резус-фактор Hemojuvelin / HFE2 Джин ORF экспрессии кДНК клона плазмидыCG90061-UTRBS13340
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Фон

Hemojuvelin, also known as HFE2, is a membrane-bound and soluble protein which belongs to the repulsive guidance molecule (RGM) family. It is known that RGMs function through Neogenin, a homologue of the Netrin receptor deleted in colon cancer. In mammals, RGM family consists of three glycoproteins which have discrete expression patterns and functions (RGM-A, RGM-B, and RGM-C). Hemojuvelin is expressed in adult and fetal liver, heart, and skeletal muscle. Hemojuvelin acts as a bone morphogenetic protein (BMP) coreceptor. Enhancement of BMP signaling regulates hepcidin (HAMP) expression and iron metabolism. It plays a key role in iron metabolism. Hemojuvelin represents the cellular receptor for hepcidin. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. Defects in hemojuvelin are the cause of hemochromatosis type 2A, also known as juvenile hemochromatosis (JH).

Ссылки
  • Papanikolaou G, et al. (2004) Mutations in HFE2 cause iron overload in chromosome 1q-linked juvenile hemochromatosis. Nat Genet. 36(1):77-82.
  • Babitt JL, et al. (2006) Bone morphogenetic protein signaling by hemojuvelin regulates hepcidin expression. Nat Genet. 38(5):531-9.
  • Zhang AS, et al. (2008) Neogenin-mediated hemojuvelin shedding occurs after hemojuvelin traffics to the plasma membrane. J Biol Chem. 283(25):17494-502.
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    Каталог: CG90061-CM
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