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Резус-фактор CD8B / P37 / LEU2 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

    ПаспортОбзорыСвязанные продуктыПротоколы
    Яванский макак CD8B Информация о продукте «Клон cDNA»
    Gene_bank_ref_id:XR_010580.2
    Размер кДНК:624bp
    Описание кДНК:Full length Clone DNA of Macaca mulatta (Rhesus monkey) t-cell surface glycoprotein CD8 beta chain-like with C terminal Flag tag.
    Синоним гена:CD8B
    Виды:Rhesus
    переносчик:pCMV3-C-FLAG
    Plasmid:
    Участок рестрикции:
    Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    Описание последовательности:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Склад:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    Резус-фактор CD8B / P37 / LEU2 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
    Резус-фактор CD8B / P37 / LEU2 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаCG90178-ACGRBS15400
    Резус-фактор CD8B / P37 / LEU2 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаCG90178-ACRRBS15400
    Резус-фактор CD8B / P37 / LEU2 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаCG90178-CFRBS13340
    Резус-фактор CD8B / P37 / LEU2 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаCG90178-CHRBS13340
    Резус-фактор CD8B / P37 / LEU2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаCG90178-CMRBS13340
    Резус-фактор CD8B / P37 / LEU2 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаCG90178-CYRBS13340
    Резус-фактор CD8B / P37 / LEU2 Джин клон кДНК в вектор клонированияCG90178-GRBS5130
    Резус-фактор CD8B / P37 / LEU2 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаCG90178-NFRBS13340
    Резус-фактор CD8B / P37 / LEU2 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаCG90178-NHRBS13340
    Резус-фактор CD8B / P37 / LEU2 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаCG90178-NMRBS13340
    Резус-фактор CD8B / P37 / LEU2 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаCG90178-NYRBS13340
    Резус-фактор CD8B / P37 / LEU2 Джин ORF экспрессии кДНК клона плазмидыCG90178-UTRBS13340
     Узнайте больше о векторов экспрессии,
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    Фон

    CD8B (CD8b molecule), also known as P37 and LEU2, contains 1 Ig-like V-type (immunoglobulin-like) domain. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. P37 gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. CD8 is thought to play a role in the process of T-cell mediated killing.

    Ссылки
  • Leahy DJ, et al. (1992) Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 A resolution. Cell. 68(6):1145-62.
  • Gao G, et al. (2000) Molecular interactions of coreceptor CD8 and MHC class I: the molecular basis for functional coordination with the T-cell receptor. Immunol Today. 21(12):630-6.
  • Devine L, et al. (1999) Orientation of the Ig domains of CD8 alpha beta relative to MHC class I. J Immunol. 162(2):846-51.
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