After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

Резус-фактор CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Cynomolgus CD36 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001032913.1
Размер кДНК:1419bp
Описание кДНК:Full length Clone DNA of Macaca mulatta (Rhesus monkey) CD36 molecule (thrombospondin receptor) with C terminal Flag tag.
Синоним гена:CD36
Виды:Rhesus
переносчик:pCMV3-C-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Резус-фактор CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Резус-фактор CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаCG90184-ACGRBS15400
Резус-фактор CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаCG90184-ACRRBS15400
Резус-фактор CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаCG90184-CFRBS13340
Резус-фактор CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаCG90184-CHRBS13340
Резус-фактор CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаCG90184-CMRBS13340
Резус-фактор CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаCG90184-CYRBS13340
Резус-фактор CD36/SCARB3 Джин клон кДНК в вектор клонированияCG90184-GRBS5130
Резус-фактор CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаCG90184-NFRBS13340
Резус-фактор CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаCG90184-NHRBS13340
Резус-фактор CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаCG90184-NMRBS13340
Резус-фактор CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаCG90184-NYRBS13340
Резус-фактор CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмидыCG90184-UTRBS13340
 Узнайте больше о векторов экспрессии,
Product nameProduct name
Фон

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 36 (CD36), also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb), is a member of the CD system as well as the class B scavenger receptor family of cell surface proteins. CD36 can be found on the surface of many cell types in vertebrate animals and it consists of 472 amino acids and is extensively glycosylated. It is an integral membrane protein primarily serving as receptors for thrombospondin and collagen and by the erythrocytes infected with the human malaria parasite. The role of CD36 as a cell surface receptor has been extended to that of a signal transduction molecule.

Ссылки
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Greenwalt RH, et al. (1992) Membrane glycoprotein in CD36: a review of its roles in adherence, signal transduction, and transfusion medicine. The journal of the American society of hematology. 80 (5): 1105-15.
  • Size / Price
    Каталог: CG90184-CF
    Цена по прейскуранту: 
    Цена:      (You Save: )
    Размер:
    Количество:+-
    Наличие2-3 weeks
    Запрос по оптовому заказуДобавить в корзину
    Contact Us
    Изображения
        Недавно просмотренные товары
          All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
          Внимание: все продукты «ПРЕДНАЗНАЧЕНЫ ИСКЛЮЧИТЕЛЬНО ДЛЯ ИССЛЕДОВАНИЙ И НЕ ПРЕДНАЗНАЧЕНЫ ДЛЯ ДИАГНОСТИКИ ИЛИ ТЕРАПЕВТИЧЕСКОГО ЛЕЧЕНИЯ».