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Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Cynomolgus CD27 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:XM_001104337.2
Размер кДНК:783bp
Описание кДНК:Full length Clone DNA of Macaca mulatta (Rhesus monkey) CD27 antigen-like with C terminal Myc tag.
Синоним гена:CD27
Виды:Rhesus
переносчик:pCMV3-C-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка on other vectors
Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаCG90049-ACGRBS15400
Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаCG90049-ACRRBS15400
Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаCG90049-CFRBS13340
Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаCG90049-CHRBS13340
Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаCG90049-CMRBS13340
Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаCG90049-CYRBS13340
Резус-фактор CD27/TNFRSF7 Джин клон кДНК в вектор клонированияCG90049-GRBS5130
Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаCG90049-G-FRBS13340
Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаCG90049-NFRBS13340
Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаCG90049-NHRBS13340
Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаCG90049-NMRBS13340
Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаCG90049-NYRBS13340
Резус-фактор CD27/TNFRSF7 Джин ORF экспрессии кДНК клона плазмидыCG90049-UTRBS13340
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Фон

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.

Ссылки
  • Drner T, et al. (2004) Correlation of circulating CD27 high plasma cells and disease activity in systemic lupus erythematosus. Lupus. 13(5): 283-9.
  • Sahota SS, et al. (2009) CD27 in defining memory B-cell origins in Waldenstrm's macroglobulinemia. Clin Lymphoma Myeloma. 9(1): 33-5.
  • Jiang J, et al. (2010) Reduced CD27 expression on antigen-specific CD4+ T cells correlates with persistent active tuberculosis. J Clin Immunol. 30(4): 566-73.
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    Каталог: CG90049-CM
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    Наличие2-3 weeks
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