|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Canine IL18 ORF mammalian expression plasmid, C-GFPSpark tag||DG70020-ACG|
|Canine IL18 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70020-ACR|
|Canine IL18 ORF mammalian expression plasmid, C-Flag tag||DG70020-CF|
|Canine IL18 ORF mammalian expression plasmid, C-His tag||DG70020-CH|
|Canine IL18 ORF mammalian expression plasmid, C-Myc tag||DG70020-CM|
|Canine IL18 ORF mammalian expression plasmid, C-HA tag||DG70020-CY|
|Canine IL18 Gene cDNA clone plasmid||DG70020-G|
|Canine IL18 ORF mammalian expression plasmid, N-Flag tag||DG70020-NF|
|Canine IL18 ORF mammalian expression plasmid, N-His tag||DG70020-NH|
|Canine IL18 ORF mammalian expression plasmid, N-Myc tag||DG70020-NM|
|Canine IL18 ORF mammalian expression plasmid, N-HA tag||DG70020-NY|
|Canine IL18 natural ORF mammalian expression plasmid||DG70020-UT|
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Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.