Быстрый заказ

Крыса Cathepsin D/CTSD Джин клон кДНК в вектор клонирования

  • Rat CTSD Gene cDNA Clone (full-length ORF Clone)
ПаспортОбзорыСвязанные продуктыПротоколы
Крыса CTSD Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_134334.2
Размер кДНК:1224bp
Описание кДНК:Full length Clone DNA of Rattus norvegicus cathepsin D
Синоним гена:Ctsd
Виды:Rat
переносчик:pUC19 Vector
Plasmid:pUC19-ratCTSD
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 837T/C not causing the amino acid variation;470C/T(S157L).
Sequencing primers:M13-47 and RV-M
( We provide with CTSD qPCR primers for gene expression analysis, RP301273 )
Promoter:
Application:
Antibiotic in E.coli:Ampicillin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Крыса CTSD Gene Plasmid Map
Rat CTSD Gene cDNA Clone (full-length ORF Clone)
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Крыса Cathepsin D/CTSD Джин клон кДНК в вектор клонирования on other vectors
Крыса Cathepsin D/CTSD Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаRG81328-ACGRBS15400
Крыса Cathepsin D/CTSD Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаRG81328-ACRRBS15400
Крыса Cathepsin D/CTSD Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаRG81328-CFRBS13340
Крыса Cathepsin D/CTSD Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаRG81328-CHRBS13340
Крыса Cathepsin D/CTSD Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаRG81328-CMRBS13340
Крыса Cathepsin D/CTSD Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаRG81328-CYRBS13340
Крыса Cathepsin D/CTSD Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаRG81328-NFRBS13340
Крыса Cathepsin D/CTSD Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаRG81328-NHRBS13340
Крыса Cathepsin D/CTSD Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаRG81328-NMRBS13340
Крыса Cathepsin D/CTSD Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаRG81328-NYRBS13340
Крыса Cathepsin D/CTSD Джин клон кДНК в вектор клонированияRG81328-URBS5130
Крыса Cathepsin D/CTSD Джин ORF экспрессии кДНК клона плазмидыRG81328-UTRBS13340
 Узнайте больше о векторов экспрессии,
Product nameProduct name
Фон

Cathepsin D (CTSD), a well known lysosomal aspartyl protease and belongs to the peptidase C1 family, which is a normal and major component of lysosomes, and is found in almost all cells and tissues of mammals. Its mostly described function is intracellular catabolism in lysosomal compartments, other physiological effect include hormone and antigen processing. Cathepsin D has a specificity similar to but narrower than that of pepsin A. Cathepsin D plays an important role in the degradation of proteins, the generation of bioactive proteins, antigen processing, etc. Among different role in cell physiology, a new function of this enzyme is examined. Cathepsin D is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis. In addition, CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases.

Ссылки
  • Fusek M, et al. (2005) Dual role of cathepsin D: ligand and protease. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 149(1): 43-50.
  • Minarowska A, et al. (2007) Regulatory role of cathepsin D in apoptosis. Folia Histochem Cytobiol. 45(3): 159-63.
  • Zaidi N, et al. (2008) Cathepsin D: a cellular roadmap. Biochem Biophys Res Commun. 376(1): 5-9.
  • Size / Price
    Каталог: RG81328-U
    Цена по прейскуранту: 
    Цена:      (You Save: )

    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Внимание: все продукты «ПРЕДНАЗНАЧЕНЫ ИСКЛЮЧИТЕЛЬНО ДЛЯ ИССЛЕДОВАНИЙ И НЕ ПРЕДНАЗНАЧЕНЫ ДЛЯ ДИАГНОСТИКИ ИЛИ ТЕРАПЕВТИЧЕСКОГО ЛЕЧЕНИЯ».