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Крыса ARG1/Arginase 1 Джин клон кДНК в вектор клонирования

ПаспортОбзорыСвязанные продуктыПротоколы
Rat ARG1 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_017134.3
Размер кДНК:972bp
Описание кДНК:Full length Clone DNA of Rattus norvegicus arginase 1.
Синоним гена:Arg1
Виды:Rat
переносчик:pUC19 Vector
Plasmid:cpUC19-ratArg1
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 146A>G(D49G) and 836A>G(N279S).
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Rat ARG1 Gene Plasmid Map
Rat ARG1 Gene cDNA Clone (full-length ORF Clone)
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Крыса ARG1/Arginase 1 Джин клон кДНК в вектор клонирования on other vectors
Крыса ARG1/Arginase 1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаRG81309-ACGRBS15400
Крыса ARG1/Arginase 1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаRG81309-ACRRBS15400
Крыса ARG1/Arginase 1 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаRG81309-ANGRBS15400
Крыса ARG1/Arginase 1 Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаRG81309-ANRRBS15400
Крыса ARG1/Arginase 1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаRG81309-CFRBS13340
Крыса ARG1/Arginase 1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаRG81309-CHRBS13340
Крыса ARG1/Arginase 1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаRG81309-CMRBS13340
Крыса ARG1/Arginase 1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаRG81309-CYRBS13340
Крыса ARG1/Arginase 1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаRG81309-NFRBS13340
Крыса ARG1/Arginase 1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаRG81309-NHRBS13340
Крыса ARG1/Arginase 1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаRG81309-NMRBS13340
Крыса ARG1/Arginase 1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаRG81309-NYRBS13340
Крыса ARG1/Arginase 1 Джин клон кДНК в вектор клонированияRG81309-URBS5130
Крыса ARG1/Arginase 1 Джин ORF экспрессии кДНК клона плазмидыRG81309-UTRBS13340
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Product nameProduct name
Фон

Arginase is the focal enzyme of the urea cycle hydrolysing L-arginine to urea and L-ornithine. Emerging studies have identified arginase in the vasculature and have implicated this enzyme in the regulation of nitric oxide (NO) synthesis and the development of vascular disease. Arginase also redirects the metabolism of L-arginine to L-ornithine and the formation of polyamines and L-proline, which are essential for smooth muscle cell growth and collagen synthesis. Arginase is encoded by two recently discovered genes (Arginase I and Arginase II). In most mammals, Arginase 1 (ARG1) also known as Arginase, liver, which functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain.

Ссылки
  • Durante W, et al. (2007) Arginase: a critical regulator of nitric oxide synthesis and vascular function. Clin Exp Pharmacol Physiol. 34(9): 906-11.
  • Waddington SN. (2002) Arginase in glomerulonephritis. Kidney Int. 61(3): 876-81.
  • Morris SM. (2002). Regulation of enzymes of the urea cycle and arginine metabolism. Annual review of nutrition. 22 (1): 87-105.
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    Каталог: RG81309-U
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