After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

Человек Acetylcholinesterase transcript variant E4-E6 Джин клон кДНК в вектор клонирования

ПаспортОбзорыСвязанные продуктыПротоколы
Human ACHE Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_000665.3
Размер кДНК:1845bp
Описание кДНК:Full length Clone DNA of Homo sapiens acetylcholinesterase.
Синоним гена:YT, ACEE, ARACHE, N-ACHE
Виды:Human
переносчик:pGEM-T Vector
Plasmid:pGEM-ACHE-E4-E6
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Человек Acetylcholinesterase transcript variant E4-E6 Джин клон кДНК в вектор клонирования on other vectors
Человек Acetylcholinesterase transcript variant E4-E6 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG15847-ACGRBS16760
Человек Acetylcholinesterase transcript variant E4-E6 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG15847-ACRRBS16760
Человек Acetylcholinesterase transcript variant E4-E6 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG15847-CFRBS14710
Человек Acetylcholinesterase transcript variant E4-E6 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG15847-CHRBS14710
Человек Acetylcholinesterase transcript variant E4-E6 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG15847-CMRBS14710
Человек Acetylcholinesterase transcript variant E4-E6 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG15847-CYRBS14710
Человек Acetylcholinesterase transcript variant E4-E6 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG15847-NFRBS14710
Человек Acetylcholinesterase transcript variant E4-E6 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG15847-NHRBS14710
Человек Acetylcholinesterase transcript variant E4-E6 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG15847-NMRBS14710
Человек Acetylcholinesterase transcript variant E4-E6 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG15847-NYRBS14710
Человек Acetylcholinesterase transcript variant E4-E6 Джин ORF экспрессии кДНК клона плазмидыHG15847-UTRBS14710
 Узнайте больше о векторов экспрессии,
Product nameProduct name
Фон

Acetylcholinesterase, also known as ACHE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. Acetylcholinesterase plays a crucial role in nerve impulse transmission at cholinergic synapses by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE appears to be a potential therapeutic target at muscle injuries including organophosphate myopathy. It is an externally oriented membrane-bound enzyme and its main physiological role is termination of chemical transmission at cholinergic synapses and secretory organs by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE plays important roles in the cholinergic system, and its dysregulation is involved in a variety of human diseases. ACHE was significantly down-regulated in the cancerous tissues of 69.2% of hepatocellular carcinoma (HCC) patients, and the low ACHE expression in HCC was correlated with tumor aggressiveness, an elevated risk of postoperative recurrence, and a low survival rate. Both the recombinant ACHE protein and the enhanced expression of ACHE significantly inhibited HCC cell growth in vitro and tumorigenicity in vivo. ACHE as a tumor growth suppressor in regulating cell proliferation, the relevant signaling pathways, and the drug sensitivity of HCC cells. Thus, ACHE is a promising independent prognostic predictor for HCC recurrence and the survival of HCC patients. ACHE is responsible for the hydrolysis of acetylcholine in the nervous system. It is inhibited by organophosphate and carbamate pesticides. However, this enzyme is only slightly inhibited by organophosphorothionates.

Ссылки
  • Zhao Y, et al. (2011) Acetylcholinesterase, a key prognostic predictor for hepatocellular carcinoma, suppresses cell growth and induces chemosensitization. Hepatology. 53(2): 493-503.
  • Roepcke CB, et al. (2010) Analysis of phosphorothionate pesticides using a chloroperoxidase pretreatment and acetylcholinesterase biosensor detection. J Agric Food Chem. 58(15): 8748-56.
  • Zaheer-ul-Haq, et al. (2010) Benchmarking docking and scoring protocol for the identification of potential acetylcholinesterase inhibitors. J Mol Graph Model. 28(8): 870-82.
  • Pegan K, et al. (2010) Acetylcholinesterase is involved in apoptosis in the precursors of human muscle regeneration. Chem Biol Interact. 187(1-3): 96-100.
  • Size / Price
    Каталог: HG15847-G
    Цена по прейскуранту: 
    Цена:      (You Save: )
    Размер:
    Количество:+-
    НаличиеIn Stock
    Запрос по оптовому заказуДобавить в корзину
    Contact Us
    Изображения
        All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
        Внимание: все продукты «ПРЕДНАЗНАЧЕНЫ ИСКЛЮЧИТЕЛЬНО ДЛЯ ИССЛЕДОВАНИЙ И НЕ ПРЕДНАЗНАЧЕНЫ ДЛЯ ДИАГНОСТИКИ ИЛИ ТЕРАПЕВТИЧЕСКОГО ЛЕЧЕНИЯ».